Lecture 7 Transcriptome Analysis How

Question 1

Types of analyses of transcripton

Answer 1

gene up analysis- where and when gene is expressed(GFP)
Biology down-
Where genes are expressed in tissue, under conditions, in a mutant comparison(microarray analysis)

Question 2

what to take into conideration when analysing transcriptome

Answer 2

Time
range
frequency of sampling and consistency
identification of gene regulation over time
link response to phenotype or behaviour- may help

Specficity of sampling
where to sample and how(cell sorting)