Lecture 9-11 Flashcards
(12 cards)
What can’t water be used to hydrolysis peptides?
during the hydrolysis of a peptide with water the transition state is not able to be stabilized so water acts as the leaving group
How does a spectrophotometer work?
the light is put through a monochromator then the incident light is put through a cuvette and then absorbance is detected
What is rate of reaction and units?
[ ] of substrate disappearing per unit time or [ ] of product producing per until, units: mol L^-1 sec^-1
What is enzyme activity and units?
the number of moles converted per unit of time, rate multiplied by reaction volume, units: 1 micromol per min
What is specific activity and units?
moles converted per unit of time per mass of enzyme or enzyme activity / actual mass of enzyme present, units: 1 micromol mg^-1 min^-1
What is molar activity/ turnover number and units?
Specific activity x molar mass of enzyme, units: min^-1
What does a low Km indicate?
that the enzyme binds and utilizes the substrate well
What is the slope, x intercept, and y-intercept of a line weaver burk plot?
Km/V-max, -1/Km, and 1/V-max
What is competitive inhibition?
when a substrate and inhibitor compete to bind to an enzyme
What effects does competitive inhibition have on V-max and Km?
Km is increased, V-max remains the same
What is non-competitive inhibition?
The inhibitor can bind to both [E] and [ES]
What effects does non-competitive inhibition have on V-max and Km?
Km is unchanged, V-max decreases
