Lecture 9

Question 1

What is embryo transfer?

Answer 1

Collection of an embryo from a donor animal and place it into another animal

Question 2

What two techniques are involved with embryo transfer?

Answer 2

Superovulation of the donor and storage and maniopulation

Question 3

What is MOET?

Answer 3

Multiple Ovulation and Embryo Transfer

Allows females to have more offspring

Question 4

What is the risk of MOET?

Answer 4

Inbreeding

Question 5

How do you superovulate?

Answer 5

Use of hormones (FSH or PMSG) and then Prostaglandin to regress corpus luteum so that a new heat arises

Question 6

What is IVF?

Answer 6

In vitro fertilisation - production of embryos in test tubes

Question 7

When do we use IVF?

Answer 7

In older valuable donors that don’t cycle normally - last resort

Question 8

What method is used to sex semen?

Answer 8

FACS (Fluorescense Activated Cell Sorting

Question 9

What does FACS show?

Answer 9

Measures DNA content of individual sperm

Question 10

How much more DNA does the X-bearing have than the Y bearing sperm?

Answer 10

3.8% MORE