Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Lab Equipment and Safety > Lectures 1-3 > Flashcards

Lectures 1-3 Flashcards

(56 cards)

Start Studying
1
Q

Universal precautions:

A

Set of precautions that assume all patients are positive for blood borne pathogens (1985)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Body substance isolation procedures:

A

Same as universal precautions, but applies to all body fluids (1987)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Five steps of standard precautions:

A
  1. Education (training for safe handling)
  2. Hand washing
  3. PPE
  4. Decontaminate surfaces
  5. Safe handling/disposal of contaminated materials
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Isolation precautions (contact):

A

-C. diff, norovirous
-gown + gloves

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Isolation precautions (droplet):

A

> 5 microns
-mumps, influenza
-mask, goggles, face shield

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Isolation precautions (airborne):

A

< 5 microns
-tuberculosis, chicken pox
-gown, gloves, goggles, N95

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Donning PPE order:

A

-HH
-Gown
-Mask
-Eyewear
-Gloves

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Doffing PPE order:

A

-Gloves
-Gown
-HH
-Eyewear
-Mask
-HH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Proper glove removal:

A

Glove-to-glove
Skin-to-skin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Small spills:

A

< 10mL
-Cover with paper towels
-Pour disinfectant from outside in
-Wait 30 min
-Remove towels with forceps
-Repeat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Large spills:

A

> 10mL
-Hold breath and move away
-Inform others
-Wait 10-30 min
-Follow small spill procedure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Spills in centrifuge:

A

-Turn off
-Don PPE
-Wait 30 min
-Remove all broken items & place in disinfectant
-Place unbroken specimens in disinfectant
-Disinfect bowl
-Dispose of cloths
-Remove sealed bucket to BSC (unless something broke, then autoclave it all)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Spills in a BSC:

A

-Fan on
-Pour disinfectant to dilute 10 fold
-Wait 30 min

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Spill kits contain:

A

-Additional PPE
-Towels + spill sock
-Container for disposal of contaminated spill kit items
-Forceps
-Disinfectant
-Autoclavable dust pan + brush
-Heavy duty polyethylene bags
-Activated charcoal
-Acid + caustic neutralizers
-Mercury vacuum/spill kit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Leaking specimens:

A

-Rejected unless irretrievable
-Inform others
-Don PPE
-Place in tray in large ziplock & move to BSC
-Perform small spill procedure where specimen was
-In BSC use paster pipette to transfer specimen to clean container
-If requisition is contaminated place in plastic until copied
-After transfer flood tray + contents with disinfectant for several hours then autoclave

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

General chemical storage:

A

-Keep secure
-Minimize quantities kept in lab
-Use unbreakable/shatterproof containers
-Store hazard liquids where spills can be contained
-Keep large containers on low shelves
-Store liquids below eye level
-Separate chemicals by reactivity class and flammability
-Keep current inventory list

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Classes of fire:

A

-Class A: combustible solids (paper, wood)
-Class B: liquid fuels (organic solvents, oil)
-Class C: electrical
-Class D: flammable metals (Na, K)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Types of fire extinguishers:

A

-Carbon dioxide: class B+C
-A-B-C dry chemical: class A+B+C
-Water: class A
-Class D dry chemical: class D

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

PASS technique:

A

Pull
Aim
Squeeze
Sweep

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Emergency fire procedures:

A

-Alert others
-Call fire department/institutional emergency line
-Evacuate lab and close door
-Pull nearest alarm
-Exit building
-If exit not possible enter designated refuge area, close door, place wet towels around openings and inform someone of your location
-Only use extinguishers on small fires
-Never use fire blanket, only stop drop and roll

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Needle stick injuries:

A

-Clean exposed area with soap + water
-Use eyewash station on mucous membranes
-Avoid chemicals/antiseptics
-Don’t milk wound
-Allow free bleeding then cover lightly
-Identify source/patient/sample code & report to supervisor immediately
-Follow internal procedures

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Hand washing technique:

A

-Wet hands
-Apply soap
-Lather entire hands for 15sec minimum
-Dry hands
-Use paper towel to close tap
-Dispose of paper towel

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Four moments of hand hygiene:

Study These Flashcards
A
  1. Upon entering patient area
  2. Immediately before aseptic procedures
  3. Immediately after exposure risk & glove removal
  4. Upon leaving
24
Q

Eye wash procedure:

Study These Flashcards
A

-Proceed directly to station
-Activate unit
-Hold eyes open & roll back and forth to rinse entire eye
-Flush for minimum 15min
-Flush 1min before removing contact lenses, then flush 15min
-Seek medical treatment

25
Emergency shower:
-Proceed directly to station -Activate unit -Remove clothing -Flush minimum 15min -Seek medical treatment
26
Biohazard waste disposal:
-Large biohazard boxes (brutes): CONTAMINATED NON-SHARPS (visibly soiled gloves, used gauze) -Sharps containers: CONTAMINATED SHARPS (lancets, used needles) -Biohazard sharps pail: OTHER CONTAMINATED SHARPS (used glass slides, test tubes) & NON-CONTAMINATED SHARPS (broken glass) -Regular garbage for NON-CONTAMINATED NON-SHARPS (gloves, paper towel)
27
Volumetric glassware:
-Measures accurate volumes -Calibration is a lengthy process -Costs more than non-calibrated -Must be high quality, transparent, & free from irregularities/defects
28
Graduated glassware:
-Piece of glassware with calibrated markings -Measures approximate liquid volumes -Poor accuracy compared to volumetric
29
Reading the meniscus:
-Always read from the bottom -Determine how many sig figs you have -*always some uncertainty*
30
Blow out:
-Pipettes designed to have the bulb blow out all contained liquid -Determined by presence of etched rings at top
31
Volumetric pipettes:
-Deliver a fixed volume by drainage -Highly accurate -Usually 1-100mL
32
Ostwald Folin Pipettes:
-Deliver a fixed volume -Lower pear-shaped bulb -Blow out -Used for viscous fluids (blood, serum)
33
Serological pipettes:
-Graduated -Larger orifice -Drains quickly -Calibrated from tip to zero -Less accurate than volumetric & mohr -Blow out
34
Mohr pipettes:
-Tip volume is unknown -Designed to deliver multiple volumes -Not to be used for a single volume -Not blow out
35
Proper pipetting technique:
-Use bulb to draw liquid up past calibration mark -Quickly replace bulb with finger -Wipe outside of pipette -Use finger to slowly drain liquid to set the meniscus -Drain into receiving container holding receiving container at an angle -Allow fluid to drain -Determine if pipette is blow out or not
36
Cleaning pipettes:
-Place in a container of 10% bleach, tip side up -Soak minimum 30min -Rinsed 1-2hrs in tap water -Rinsed 2-3 times in distilled water -Dry in hot air oven
37
Cleaning glassware:
-Can be left to soak overnight in water + detergent until you have time to wash -Decontaminate prior to cleaning by pre-soaking in 5% bleach -After washing rinse 4-5 times in tap water then 2-3 times in distilled water
38
Disposable transfer pipettes:
-Most commonly used transfer pipette -One piece -Safe method for liquid transfer -Sizes vary -Can be sterile or non-sterile
39
Automatic pipettes:
-Used for rapid, repetitive measurements of small volumes, usually in micro litres -You set the volume (within range) -Uses disposable plastic tips -No need to wipe (non-wettable tips) -Most auto-eject tips -Frequent calibration required
40
Disadvantages of plastic ware:
-Leaching into solutions -Some permeability to water vapour -Absorption of dyes, stains, proteins -Affected by oxidizing agents -Some soluble in toluene, chloroform -Affected by sunlight -Organic solvents can evaporate through -Cannot be heated
41
Polyolefins:
-Used for polyethylene & polypropylene -Most widely used plastic -No reaction with most chemicals -Reacts with strong oxidizers at high temps
42
Polycarbonate resin:
-Physically strong -Heat & scratch resistant -Glass-like transparency
43
Tygon:
-Clear -Non-toxic -Flexible -Unreactive -Can be autoclaved -Plastic tubing
44
Teflon fluorocarbon resin:
-Resists most chemicals -Withstands -270-255 degrees C -Expensive -Bottles, beakers, cylinders
45
Polystyrene:
-Vinyl polymer -Inexpensive -Mostly disposable -Second most common
46
Borosilicate glass:
-High thermal resistance -Low alkali -Heat up to 600C -Shock resistant -Pyrex, Kimax, Exax, Vycor, Corex -Beakers, flasks, pipettes
47
Alumina glass:
-High silicate content -Chemically strengthened -Radiation, heat, & acid resistant -Good precision -Microscopes, lenses, cuvettes
48
Acid/Alkali resistant glass:
-Resists corrosion & thermal shock -Boron free. weaker than borosilicate -"Soft glass" -Not resistant to thermal shock -Not very common
49
Flint glass:
-Oxides of silicon, Ca, Na -Clear -Cheapest -No heat or thermal shock resistance -Used for disposability
50
Low actinic glass:
-Brown or amber -Stores light-sensitive materials -Can be any type of glass
51
Labeling containers:
-Supplier labels (product identifier, initial supplier identifier, pictogram(s), signal word, hazard statements, precautionary statements, supplemental info, easily displayed) -Workplace labels (required when hazard is transferred, name, safe handling, SDS reference)
52
Calibration of volumetric glassware:
-Determines exact volume -Marked in graduations -Checked against specifications -Performed by weight with distilled water
53
Preparing reagents in a volumetric flask:
-Always add chemical to water -Use solvent to rinse all solute from weighing vessel if solute is solid -Add liquid solvent to flask to just below neck -Stopper flask & mix by inversion -Add solvent with transfer pipette drowse up to calibration mark -Ensure fluid is at room temp first
54
Absolute and relative uncertainty:
-Absolute: X plus or minus Y -Relative: absolute divided by measurement time 100%
55
Accuracy:
-Closeness to true volume -Can describe technique -Can describe glassware -Lower uncertainty = higher accuracy
56
Precision:
-Reproducibility -Technique -Getting same results from same test = precision -Glassware (if a pipette always delivers same volume) -Not necessarily accurate (multiple identical but inaccurate results

Lab Equipment and Safety (3 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions