Legionella pneumophila

Question 1

Pathogen

Answer 1

• G- rod
• obigative anerobe
• not easily stained
• Intracellular pathogen (mimicry)
• Mobile
• Two phases:
  1) . Infectious phase (entrance & death) –> has flagella
  2) . Repicative (w/in cell) —> no flagella

Question 2

Encounter

Answer 2

fresh water or soil
intcl pararsite of 13 & 2 ciliated protozoa

Question 3

Entry

Answer 3

• inhalation of aerosole contam. water

Question 4

Spread

Answer 4

• point source spread
• NO person —> person

Question 5

Multiplication

Answer 5

• colonizes aveolar macrophages

Question 6

Damage

Answer 6

• Pneumonia —-> host inflam response
• eldery & immunocompromised

Question 7

DX

Answer 7

• urine Ag test
• culture sputum (specialized media)

Question 8

TX

Answer 8

• Antibiotics: macrolides, tetracyclines, fluoroquinolones

Question 9

PV

Answer 9

• moniter water system

Question 10

Mechinism of Evasion

Answer 10

• Type IV secretion system releases effector protiens:
• recruits vesicles from mitochondria & ER, bacteria becomes “self”-recognized
• also prevents fusion with the lysosome
• Feeds off materials need for cyctoplasm
• LCV uses all of cells resource then kills cell

Question 11

Virulence Factors:
Type IV Secretion System

Answer 11

• secrets effector protiens
• requires all 26 genes for function
• -1 gene —-> no intcl growth
• AKA: Dot/Icm

Question 12

Virulence Factors:
Effector Proteins

Answer 12

• ~100 secrected via T4SS
• 20 varifible by immunoflourecent microscopy
• 80 predicted via bioinformatic analysis
• vary widley in fuction
• some host specific
• deletion of single factor = no effect on intcl growth