Les 1, cel culturen Flashcards

1
Q

wat voor type cel is RAW?

A

Muis macrofaag - - adherent

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2
Q

wat voor type cel is L929 ?

A

muis fibroblast

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3
Q

Wat voor type cel is DO11.10?

A

Muis T-cel

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4
Q

Wat zit er in celkweek medium? (7)

A
  • Energy bron → glucose
  • aminozuren
  • vitamine
  • Antibiotica
  • pH-indicator → phenol rood
  • inorganische zouten → osmotische regulatie
  • serum FCS (fetal kalf serum)→ bron groei factoren, hormonen, mineralen, lipiden …
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5
Q

Wat is confluency?

A

hoe vol het opp is
geeft info over de groei fase van de cellen

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6
Q

hoe passeer je adherende cellen?

A

schrapen en Trypsin

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7
Q

welke contaminatie komt het meest voor?

A

bacteriën, schimmels en gist

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8
Q

Hoe kan contaminatie komen?

A

water bad, CO2 incubator, researcher

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