Lesson 2 Protein Denaturation Flashcards
(14 cards)
What is Protein Denaturation?
- Any modification in confirmation not accompanied by rupture of peptide bonds
- Involves the disruption and possible destruction of both the secondary and tertiary structures
Types of chemical bonds that are targeted by denaturing agents (SECONDARY STRUCTURE)
In secondary structures – hydrogen bonds are disrupted
Types of chemical bonds that are targeted by denaturing agents (SECONDARY STRUCTURE)
In secondary structures – hydrogen bonds are disrupted
In tertiary structure there are four types of bonding interactions between “side
chains” including:
❏ hydrogen bonding
❏ salt bridges
❏ disulfide bonds
❏ and non-polar hydrophobic
interactions
7 Effects of Denaturation
❏ Decreased solubility
❏ Altered water binding capacity
❏ Loss of biological activity
❏ Destruction of toxins
❏ Improved digestibility
❏ Increased intrinsic viscosity
❏ Inability to crystallize
Heat disrupts what bonds?
disrupt hydrogen bonds and non-polar hydrophobic interactions
Heat increases the _______ and causes the molecules to vibrate so _______ and ______ that the bonds are disrupted
kinetic energy, rapidly and violently
Disrupts hydrogen bonding
Alcohol
Alcohol denatures both what structures?
both secondary and tertiary structures
Since salts are ____, they disrupt ____________ in proteins or replaces _______ with ________
ionic, salt bridges, one metal with another
Metals are also reducing agents/oxidizing agents that can __________
disrupt the disulfide bonds in protein structure
acids and bases disrupt salt bridges held together by _____ that can affect the ___________
ionic charges, environmental pH of proteins.
Alkaloidal reagents have a high
molecular weight anions
Alkaloidal reagents: The negative charge of these anions counteracts the positive charge of the _______ disrupting _____
amino group in proteins, salt bridges
