Lesson 2: Reactions and Stability of Proteins Post Lab

Question 1

disrupts hydrogen bonds of secondary and tertiary protein structure while the primary structure remains unaffected

Answer 1

Heat Denaturation

Question 2

Can cause change in the protein bonds

Answer 2

Temperature
pH
Substance

Question 3

increase in kinetic energy of the atoms in the structure

Answer 3

Heat Denaturation

Question 4

Heat Denaturation Positive Result:

Answer 4

formation of coagulum

Question 5

coagulable proteins

Answer 5

Albumin and globulin

Question 6

non-coagulable proteins

Answer 6

Gelatine and peptone

Question 7

Heat Denaturation Disrupted bonds:

Answer 7

hydrogen bonds of secondary and tertiary protein structure

Question 8

charges are balanced →
↓ repulsive electrostatic forces; ↑ attraction forces

Answer 8

Isoelectric Precipitation

Question 9

Isoelectric Precipitation Positive Result:

Answer 9

precipitation

Question 10

Isoelectric Precipitation Disrupted bonds:

Answer 10

salt bridges and hydrogen bond

Question 11

altering the ionization (charge) of the carboxylate and ammonium ion groups

Answer 11

Concentrated Mineral Acids

Question 12

opposite charges ______ while same charges ______ and neutral ______

Answer 12

attract; repel; not reaction

Question 13

altering the ionization (charge) of the carboxylate and ammonium ion groups

Answer 13

Concentrated Mineral Acids

Question 14

Concentrated Mineral Acids Positive Result:

Answer 14

precipitation

Question 15

Concentrated Mineral Acids Disrupted Bonds:

Answer 15

salt linkages and hydrogen bonds

Question 16

Carboxylate ion:

Answer 16

Aspartic acid and glutamic acid

Question 17

Ammonium ion:

Answer 17

lysine and arginine

Question 18

“reversible”
Water molecules bound with proteins are
more attracted to salt

Answer 18

Salting Out

Question 19

Salting Out Positive Result

Answer 19

: precipitation

Question 20

Salting Out Disrupted bonds:

Answer 20

ionizable groups

Question 21

half-saturation

Answer 21

Globulins

Question 22

full saturation.

Answer 22

Albumins

Question 23

• high solubility;
  reversible water binding process

Answer 23

Ammonium Sulphate

Question 24

metallic ions + anionic form of proteins
(carboxylate group (-COO-) → metal proteinates

Answer 24

Salts of Heavy Metals

Question 25

Salts of Heavy Metals Reactive group:

Answer 25

salt bridges (carboxylate group) and sulfhydryl groups

Question 26

reagents counteracts the positive charge of the amino group in proteins

Answer 26

Alkaloidal Reagents

Question 27

Alkaloidal Reagents Reactive group:

Answer 27

salt linkages (amino group)

Question 28

Organic Solvents Reactive group:

Answer 28

hydrophobic interaction between R groups of amino acids; hydrogen bonds between water and polar R groups of amino acid

Question 29

Organic Solvents Positive Result:

Answer 29

precipitation leading to flocculum