Lesson 2 Slide Preparation

Question 1

A process wherein the specimen are preserved in a” life-like” state

Answer 1

Fixation

Question 2

is the most basic technique, ideal for observing hair, feathers, airborne particles such as pollens and dust

Answer 2

Dry Mount

Question 3

is used for aquatic samples, living organisms and natural observation

Answer 3

Wet Mount

Question 4

How to prepare slide?

Answer 4

Place-Position-Place-Lower-Remove
● Place a drop of fluid in the center of the slide ● Position sample on liquid, using tweezers ● At an angle, place one side of the cover slip against the slide making contact with outer edge of the liquid drop ● Lower the cover slowly, avoiding air bubbles (if bubbles are present, tap gently the cover slip using the eraser end of a pencil to move

Question 5

require two or more flat, plain slides, cover slips, pipette and tissue paper

Answer 5

Smear Slides

Question 6

Is designed for soft samples

Answer 6

Squash Slides

Question 7

A variety of methods exist for staining microscope slides, including non-vital or in vitro stains

Answer 7

Staining

Question 8

especially useful in the fields of histology, virology and pathology, allowing researchers to study and diagnose diseases

Answer 8

Stains