Lesson 6: Infiltration and Embedding

Question 1

Process whereby the clearing agent is completely removed from the tissue and replaced by a medium that will completely fill out the tissue cavities

Answer 1

Infiltration

Question 2

A good infiltrating medium is ideally molten between ___ to ___

Answer 2

30C to 60C

Question 2

A good infiltrating medium should have a melting point of below ___ in the pure state

Answer 2

Below 60C

Question 2

2 Types of Infiltration Media

Answer 2

Water Miscible
Water Immiscible

Question 3

Overheated paraffin makes the specimen _______

Answer 3

brittle

Question 3

3 Types of Water Miscible Media

Answer 3

Gelatin
Carbowax
Agar

Question 4

2 Types of Water Miscible Media

Answer 4

Paraffin Wax
Paraplast

Question 4

Also known as blocking/casting

Answer 4

Embedding

Question 5

True or False

Paraffin processing is recommended for fatty tissues

Answer 5

False, it is not recommended

Question 5

Types of Embedding Mold (4)

Answer 5

Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Ring & Base Mold
Disposable Embedding Mold

Question 6

This will promote retention of the clearing agent

Answer 6

Inadequate impregnation

Question 6

Excessive tissue shrinkage and hardening is caused by ____________ impregnation

Answer 6

Prolonged

Question 7

3 Types of Disposable Embedding Mold

Answer 7

Peel-away
Plastic Ice Tray
Paper Boat

Question 7

It is a mold that consists of 2-L shaped strips of heavy brass or metal

Mold is adjustable to make different size of blocks

Answer 7

Leuckhart Mold

Question 8

It is a mold made up of a series of interlocking plates resting on a flat metal base

Advantage: Embedding more specimens at a time

Answer 8

Compound Embedding Unit

Question 9

It is a mold that consists of a special stainless steel base mold fitted with a plastic embedding ring, which serves as the block holder during cutting

Answer 9

Plastic Embedding Ring

Question 10

A disposable thin plastic embedding mold. They are simply peeled off one at a time, as soon as the wax has solidified, giving perfect even block without trimming

Answer 10

Peel Away

Question 11

May be recommended by busy routine laboratories. Each compartment may be utilized for embedding “one tissue block”

Answer 11

Plastic Ice Trays

Question 12

A type of disposable mold:

Oldest method
Advantage of being cheap and easy to make

Answer 12

Paper Boat Method

Question 13

Melting Point of paraffin wax

Answer 13

40-60C

Question 14

For tropical countries, hard wax having a melting point of __ to __ is suitable

Answer 14

58-60C

Question 14

A synthetic wax substitute similar to paraplast with a melting point of 56-58C

Answer 14

Embeddol

Question 14

Most commonly used tissue embedding medium

Answer 14

Paraffin Wax

Question 15

Melting Point of Paraplast

Answer 15

56-57C

Question 15

This is a mixture of purified paraffin and plastic

Answer 15

Paraplast

Question 15

Easy to make the serial sections of 4 microns due to its elasticity

Answer 15

Paraplast

Question 16

It is less brittle and less compressible than paraplast

Answer 16

Embeddol

Question 16

A semi-synthetic wax recommended for embedding "eyes"

Answer 16

Bioloid

Question 17

A product of paraffin, containing rubber, with the same property as a paraplast

Answer 17

Tissue Mat

Question 17

Harder than paraffin but has a low melting point of 46-48C

Answer 17

Ester Wax

Question 18

A mixture of paraffin and rubber

Answer 18

Tissue Mate

Question 19

It is rarely used except when dehydration is to be avoided and when tissues are subjected to histochemical and enzyme studies

Answer 19

Gelatin

Question 20

Used for delicate specimens and frozen tissue sections Water-soluble Low melting point Tissues should not be more than 2-3 mm thick

Answer 20

Gelatin

Question 21

Gelatin contains this solution that serves to prevent the "growth of molds"

Answer 21

1% phenol

Question 21

A purified form of nitrocellulose, soluble in many solvents

Answer 21

Celloidin

Question 22

This is suitable for specimens with large hollow cavities Used for hard and dense tissues Recommended for processing "Neurological Tissues"

Answer 22

Celloidin

Question 23

"Light loose texture materials" are best stirred up while the paraffin wax is ______, if not followed it may not aggregate in the center of the mold

Answer 23

warm