Ligand binding

Question 1

What does multiple identical binding sites mean?

Answer 1

Several ligand binding sites with the same affinity

Question 2

What odes multiple non-identical independent binding sites mean?

Answer 2

The binding sistes are independent (as if they were identical) but have different affinity

Question 3

What does multiple dependent binding sites mean and what is another name for it?

Answer 3

Binding to one site will affect affinity to the others - aka cooperativity

Question 4

What are the four types of ligands?

Answer 4

• Cofactor
• Metal
• Substrate
• Inhibitor

Question 5

How is X-ray used for detecting ligand binding?

Answer 5

Creates a snapshot of how the ligand will bind to the protein

Question 6

How is NMR used for detecting ligand binding?

Answer 6

Shows how a specific aa will be affected by a ligand