Ligand Binding Flashcards
antibody ligand
antigen
antibodies are built out of many copies of one type of domain - an _____
immunoglobulin fold
programmed recombination
mixes a family of sequences and somatic hypermutation causes point mutations to yield aminoa cid sequence diversity at the antigen binding site
structural scaffold of antibody
supports binding sites so that overall protein can fold regardless of variable region
kd equation
[P][L]/[P*L]
ka equation (also keq)
[P*L]/[P][L]
specificity equations
[ka1]/[ka2]
[kd2]/kd1]
k1 rate constant is
2nd order
k-1 rate constant is
1st order
v =
M/s
k1 unites
M-1s-1
k-1 unites
s-1
kd in terms of k0ff kon
[koff]/[kon]
kd in terms of pl
[P][L]/[P*L]
steeper slope kinetic meaning
tighter bind, lower kd
flatter slope kinetic meaning
weaker bind, higher kd
role of polypeptide portion of Mb
prevent iron oxidation from fe2 to fe3
modulate affinity for oxygen by making a pocket and interacting
protein favors which conformation
bent conformation where distal his (e7) can form h bond
number of mb proteins remains
constant
fractional saturation
theta, fraction of ligand-binding sites in P occupied by ligand
theta in terms of l and kd
[L]/kd+[L]
theta in terms of words
[binding sites occupied]/[total binding sites]
theta for partial pressure
pO2/P50 + pO2
keq
e^(-deltaG/RT)
T is in Kelvin
