light-dependent and independent reactions & succession Flashcards
(47 cards)
what is the role of NADP in the light-dependent reaction
coenzyme
transfers hydogen atoms from one molecule to another
when NADP gains a H it becomes reduced NADP or NADPH
either oxidises other molecules by gaining H or reduces other molecules by giving them H
what does the light-dependent reaction use and produce
uses H2O and light energy
produced O as a waste product and transfers light energy into chemical energy to be used in calvin cycle
what does the calvin cycle/ light independent reaction require to function
ATP and NADPH from light dependent reaction
and CO2
what is produced from the calvin cycle
carbohydrates
NADP and ADP from light-independent reactions can be used
what is photophosphorylation
happens in light-dependent reaction on thylakoid membrane
ADP-> ATP by addition of phosphate using light energy
what is chemiosmosis
movement of ions (usually H+) across a membrane leading to production of ATP
Driven by a gradient across the membrane
what is Pi
phosphate ion
does condensation or hydrolysis produce water as a byproduct
condensation
hydrolysis includes addition of water
what is produced by non-cyclic phosphorylation
NADPH and ATP
What is produced by cyclic photophosphorylation
just ATP
What do both cyclic and non-cyclic PHOTOphosphorylation involve (to do with proteins and electrons)
-transfer of electrons between membrane proteins down electron transport chain, releasing energy as theyre passed along (chemiosmosis)
what is a pigment
light-absorbing molecules
can harvest light energy
what is a photosystem
complexes of proteins and pigments and a pair of chlorophyll molecules at its core
2 types of photosystem, I and II
in 2 water gets split by light energy (photolysis) releasing electrons, H+ and oxygen
What happens non-cyclic photophosphorylation (NADPH AND ATP produced)
electrons excited by light energy, leave hydrogen
hydrogen picked up by NADP
passed onto neighbouring proteins, energy realeased?
answer: involves both photosystems 1 and 2
electrons removed from water and passed onto NADP
how many times does water get removed from water and does light get absorbed throughout non-cyclic photophosphorylation
light gets absorbed 2x once at each photosystem
which photosystem is involved in cyclic photophosphorylation
only photosystem 1
which photosystem is responsible for splitting water
photosystem 2
difference between photolysis and hydrolysis
photolysis is the splitting of a molecule by absorbing light/light energy
( water into 2 electrons,2 H+ and one oxygen atom)
hydrolysis is splitting of a molecule by addition of a water molecule
describe non-cyclical photophosphorylation
rough: creates conc gradient, electrons removed from water REPLACES ELECTRON IN PIGMENT AND ELECTRON FROM PIGMENT RELEASED AT HIGH ENERGY THEN passed between proteins on membrane surface, H+ ions released INTO THYKAKOID LUMEN creating conc gradient
H+ ions moving down conc gradient allow ATP synthase to convert ATP from ADP
answer:
what happens when light is absorbed by pigment in photosystem 2
energy transferred to pigment in core (P680) and electron in this pigment is boosted at a high energy level and passed onto an acceptor molecule
these electron are replaced by electron sfrom split water
O released as waste product
H+ ions from water go into thylakoid MEMBRANE
how does this lead to ATP synthesis?
2 high energy electrons released from pigment passed along electron transport chain, losing energy used to pump H+ ions from stroma into thylakoid SPACE (later pumped into lumen) building a gradient
H+ from splitting of water also help to build gradient
energy released from electron transport chain releases energy that pumps H+ from the stroma into the thylakoid space
as H+ ions flow down their conc gradient INTO THE STROMA they pass through ATP synthase allowing it to produce ATP from ADP (chemiosmosis)
what happens at the end of the electron transport chain
-ATP produced as H+ ions move from thylakoid lumen into the stroma (by energy released from electron transport chain pumping them from the stroma into thylakoid membrane
these ions flow through ATP synthase when they flow back into the stroma)
-electrons from photosystem 2 are passed to photosystem 1, electron accepted by NADP where it becomes reduced in stroma, NADPH produced and used in light dependent reaction
what happens at photosystem 1
light energy also hits this photosystem
-this excites another pair of electrons and they leave the photosystem
-passed along the electron transport chain
-NOW NADPH IS PRODUCED
these electrons combine with the H+ from photolysis of water and NADP+ to form NADPH
light energy hits photosystem 2
electrons in pigment excited and sent into higher orbital energy, replaced by electron from photolysis of water, H+ from split water goes into thylakoid lumen and O released as a waste product, electron passed down electron transport chain, proteins on thylakoid membrane by a series of oxidation and reduction reactions
at photosystem 1
conc gradient formed by H+ in lumen crossing the membrane and allowing ATP synthase to produce ATP with ADP and Pi
chemiosmosis
NADPH produced to be used in light independent reaction