LIPID AND LIPOPROTEIN ANALYSES

Question 1

Principle: Dehydration and oxidation of cholesterol to form a colored compound (colorimetric)
A. CHOLE MEASUREMENT
B. TRIGLY MEASUREMENT
C. LIPOPROTEIN MEASUREMENT

Answer 1

A. CHOLE MEASUREMENT

Question 2

**CHOLESTEROL **

SAMPLE SHOULD BE
A. PLASMA
B. SERUM
C. BOTH A AND B
D. EDTA

Answer 2

BOTH A AND B

Question 3

CHOLESTEROL PATIENT SHOULD HAVEA A
_ week prior testing with in a usually diet

A. 3 WEEKS
B 1 WEEK
C. 2 WEEKS

Answer 3

C. 2 WEEKS

Question 4

**SAMPLE SUBMITTED FOR CHOLESTEROL MEASUREMENT SHOULD BE **
A. NON FASTING BLOOD SAMPLE
B. FAST BLOOD SAMPLE

Answer 4

A. NON FASTING BLOOD SAMPLE

Question 5

Non-Enzymatic Method FOR CHOLESTEROL

A. ABELL KENDALL
B. CHOLESTEROL OXIDASE

Answer 5

A. ABELL KENDALL

Question 6

Principle: Dehydration and oxidation of cholesterol to form a colored compound

A. NON-EZYMATIC ABELL KENDALL
B ENZYMATIC ABELL KENDAL

Answer 6

A. NON-EZYMATIC ABELL KENDALL

Question 7

One step method
A. COLORIMETRY
B. extraction followed by colorimetry
C. extraction, saponification, and colorimetry
D.precipitation, saponification, extraction, and
colorimetry

Answer 7

A. COLORIMETRY

Question 8

**Two step method IN ABEL KENDALL **

A. COLORIMETRY
B. extraction followed by colorimetry
C. extraction, saponification, and colorimetry
D.precipitation, saponification, extraction, and
colorimetry

Answer 8

B. extraction followed by colorimetry

Question 9

3 step method IN ABEL KENDALL
A. COLORIMETRY
B. extraction followed by colorimetry
C. extraction, saponification, and colorimetry
D.precipitation, saponification, extraction, and
colorimetry

Answer 9

C. extraction, saponification, and colorimetry

Question 10

4 step method IN ABEL KENDALL
A. COLORIMETRY
B. extraction followed by colorimetry
C. extraction, saponification, and colorimetry
D.precipitation, saponification, extraction, and
colorimetry

Answer 10

D.precipitation, saponification, extraction, and
colorimetry

Question 11

Cholesteryl esters are hydrolized with

A. HEXANE
B. ALCOHOLIC KOH

Answer 11

alcoholic KOH

Question 12

Unesterified cholesterol is extracted with
A. HEXANE
B. ALCOHOLIC KOH

Answer 12

HEXANE

Question 13

** THE NON ENZYMAIC METHOD OF ABELL KENDALL IS Measured using:**

A. Liebermann-Burchard reaction
B. Brodie method

Answer 13

A. Liebermann-Burchard reaction

Question 14

NON ENZYMATIC

❖ Cholesterol + sulfuric acid + acetic anhydride→

A. BLACK
B. GREEN (CholestadienylMonosulfonicAcid)
C. RED SOLUTION . (CholestadienylDisulfonic Acid)

Answer 14

B. GREEN

Question 15

Salkowski reaction:

A. BLACK
B. GREEN
C. RED SOLUTION

Answer 15

C. RED SOLUTION
. (CholestadienylDisulfonic Acid)

Question 16

Cholesterol oxidase
A. Enzymatic Method
B. NON ENZYMATIC METHOD

Answer 16

A. Enzymatic Method

Question 17

Enzymatic Method

H2O2 + Dye –Peroxidase -> WHAT COLOR OF THE PRODUCT (Quinoneimine dye)

Answer 17

VIOLET

Question 18

Cholesterol + O2 –Cholesterol oxidase - >

A. 4-cholestenone + h2o2
B. cholesterol + fatty acid

Answer 18

A. 4-cholestenone + h2o2

Question 19

Total Cholesterol (cholesteryl ester and cholesterol) + H2O –Cholesteryl esterase ->

A. 4-cholestenone + h2o2
B. cholesterol + fatty acid

Answer 19

B. cholesterol + fatty acid

Question 20

**TRIGLYCERIDE FASTING ONLY
→ There is a strict fasting for - hours **

A.12 HRS
B 10 HS
C. 8 HRS

Answer 20

A.12 HRS

Question 21

Triglycerides with FBS (fasting blood glucose) fasting for HOW MAY hrs is accepted

A.10HRS
B. 12 HRS

Answer 21

A.10HRS

Question 22

NON ENZYMATIC FOR TRIGLY MEASUREMNT

FIRST. Triglyceride –Alcoholic KOH →

A. + Glycerol + Fatty acid
B. Formaldehyde

Answer 22

A. Glycerol + Fatty acid

Question 23

NON ENZYMATIC FOR TRIGLY MEASUREMENT

2ND. Glycerol + Periodic acid →

A. FORMALDEHYDE
B. + Glycerol + Fatty acid

Answer 23

A. FORMALDEHYDE

Question 24

**Van Handel and Zilversmith **
Formaldehyde + Chromotropic acid → Blue Solution

A, Colorimetric Method)
B. Fluorometric Method)

Answer 24

A, Colorimetric Method)

Question 25

**Hantzch Condensation ** Formaldehyde + Diacetyl acetone + NH3 → **Yellow Solution** A. COLORIMETRIC B. FLUOROMETRIC METHOD

Answer 25

B. FLUOROMETRIC METHOD

Question 26

Enzymatic Methods FOR TRIGLY A. : Glycerol Kinase B. : Abell Kendall

Answer 26

A. : Glycerol Kinase

Question 27

**ADP + PEP –Pyruvate kinase ATP+ Pyruvate Pyruvate + NADH + H+ –LD-->* Lactate* + NAD+ (340 nm)** A. PYRUVATE KINASE CHOLE B PYRUVATE KINASE TRIGLY

Answer 27

B PYRUVATE KINASE TRIGLY

Question 28

Glycerophosphate + O2 –GO → **+ Dihydroxyacetone **+ H2O2 o H2O2 + Dye –Peroxidase → Color A. PYRUVATE KINASE CHOLE B PYRUVATE KINASE TRIGLY C. GLYCEROL- PHOSPHATE OXIDASE

Answer 28

Glycerol-Phosphate Oxidase

Question 30

CDC REFERENCE METHOD: Modified Van handheld and SilversmitH A. TRIGLY B. CHOLE

Answer 30

A. TRIGLY

Question 31

**LIPOPROTEIN MEASUREMENT Preferred sample** A. SERUM SST B. EDTA

Answer 31

A. SERUM SST

Question 32

**For Research Purposes FOR LIPOPROTEIN ** A. SST SERUM B. EDTA PLASMA C EDTA SERUM

Answer 32

B. EDTA PLASMA

Question 33

# LIPOPROTEIN MEASUREMENT TRUE OR FALSE **For non-fasting sample: total cholesterol and HDL can be measured but not LDL and VLDL**

Answer 33

TRUE

Question 34

# LIPOPROTEIN **Ultracentrifugation** SOLUTIO REAGENT SG A. Potassium Bromide with a specific gravity fixed at 1.063 B.Potassium Bromide with a specific gravity fixed at 1.064

Answer 34

A. Potassium Bromide with a specific gravity fixed at 1.063

Question 35

Order from the lightest to heaviest:

Answer 35

Chylomicrons →VLDL →LDL →HDL

Question 36

COLLECTED SUPERNATE IN THE CENTRI A. HDL B LDL C. VLDL

Answer 36

HDL

Question 37

PRECIPITATE WHICH LEFT IN THE TUBE A. HDL B LDL C. VLDL

Answer 37

LDL C. VLDL

Question 38

Lipid Profile Test contains a. total cholesterol b. triglycerides c. HDL, LDL, and VLDL d. all

Answer 38

all

Question 39

**are not directly measured (not usually measured)** a. ldl and vldl b. total chole, trig, hdl

Answer 39

a. ldl and vldl

Question 40

**– for determination of LDL and VLDL conc.** a. apo B B. APO A-1

Answer 40

a. apo B

Question 41

**for determination of HDL conc.** a. apo B B. APO A-1

Answer 41

B. APO A-1