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CC1 MIDTERM - LIPIDS > LIPIDS and LIPOPROTEINS > Flashcards

LIPIDS and LIPOPROTEINS Flashcards

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1
Q

basically fats

A

lipids

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2
Q

major class of dietary lipids

A

Triglycerides

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3
Q

lipids that are solid at RT

A

Fats

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4
Q

lipids that are liquid at RT

A

Oils

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5
Q

constituent of cell membranes

A

Phospholipids

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6
Q

forms of Phospholipids with percentage

A
  • 70% Lecithin/Phosphatidyl choline
  • 20% Sphingomyelin
  • 10% Cephalin
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7
Q

precursor of steroid hormones

A

Cholesterol

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8
Q

not used for energy

A

cholesterol

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9
Q

building blocks of lipids

A

fatty acids

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10
Q

bound to the proteins which allow fats to move through the water in and out of cells

A

Lipoproteins

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11
Q

most dense to least lipoprotein

A

HDL > LDL > VLDL

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12
Q

Heaviest to lightest based on molecular weight

A

Chylomicron > VLDL > LDL > HDL

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13
Q

Components of Chylomicron

A
  • 85-92% TAG
  • 6-12% Phospholipids
  • 1-3% Cholesterol
  • 1-2% Proteins
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13
Q

this is the responsible for POST-PRANDIAL TURBIDITY of sample

A

chylomicron

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14
Q

Types of Lipoproteins

A
  • Chylomicron
  • VLDL
  • IDL
  • LDL
  • HDL
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15
Q

responsible for FASTING HYPERLIPIDEMIC TURBIDITY of sample

A

VLDL

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16
Q

Second highest TAG content

A

VLDL

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17
Q

VDLD remnant

A

IDL

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18
Q
  • transports TAGS, fats and cholesterol
  • promote the growth of atheroma
A

IDL

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19
Q

products of VLDL and IDL metabolism

A

LDL

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20
Q

most cholesterol-rich

A

LDL

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21
Q

aka BAD CHOLESTEROL

A

LDL

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22
Q

phospholipid is the main lipidic content

A

HDL

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23
Q

aka GOOD CHOLESTEROL

A

HDL

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24
synthesized in the liver and intestine
HDL
25
smallest and densest lipoprotein
HDL
26
proteins that bind lipids to form lipoprotein
Apolipoproteins
27
serve as enzyme cofactors and receptor ligands
apolipoproteins
28
LP Carried: Activator of LCAT for esterification
Apo A-1
29
LP Carried: Chylomicrons
Apo B-48
30
LP Carried: Lipemia clearing factor
Apo C-II
31
LP Carried: Activates LPL, targets TAG and removes CM after meal
Apo C-II
32
LP Carried: Increased in Alzheimer's Dx
Apo E
33
Lipid Storage Diseases: Accumulation of sphingomyelin in the bone marrow, spleen and lymph nodes
NIEMANN-PICK DISEASE
34
Lipid Storage Disease: orange or yellow discoloration of tonsils and pharynx
TANGIER’S DISEASE
35
Lipid Storage Disease: - Deficiency of hexosaminidase A - Accumulation of sphingolipids in the brain
TAY-SACH’S DISEASE
36
Lipid Storage Disease: Chylomicron-retention disease
ANDERSON’S DISEASE
37
Lipid Storage Diseases: Plant sterols absorbed and accumulated in the blood and tissues
SITOSTEROLEMIA
38
not affected by fasting
Cholesterol determination
39
Specimen used
Serum or Plasma EDTA
40
Cholesterol Measurement : Old reference method
Abell- Kendall Method
41
3 step method of Abell-Kendall
Saponification - Extraction - Colorimetry
42
end product and color of Abell-Kendall Method
Cholestadienyl monosulfuric acid , GREEN
43
Cholesterol Measurement: Current reference method
Abell, Levey, Brody Method
44
reagents used and end color
Libermann-Burchard rgt + KOH and alcoholic hexane , GREEN
45
4 step method of Sperry Method
Saponification - Extraction - Colorimetry - Purification with digitonide
46
end product and end color of Salkowski Method
Cholestadienyl disulfuric acid, RED
47
Cholesterol Measurement: most commonly used
Enzymatic Method
48
interferences of Enzymatic Method
ascorbate, hemoglobin, bilirubin
49
Cholesterol Measurement: Definitive Method
Isotope Dilution/Mass spectrometry
50
Triglycerid Measurement: Colorimetric Method
Van Handel & Zilversmit Method
51
Reagent and End Color for Van Handel & Zilversmit Method
Chromotropic acid , BLUE
52
Triglyceride Measurement: Colorimetric Method and Reference Method
Modified Van Handel & Zilversmit Method
53
Reagent and End Color for Modified Van Handle & Zilversmit Method
Sulphuric Acid and Salicylic Acid, PINK
54
Triglyceride Measurement: Fluorometric Method
Hantzsch Method
55
Reagent and End Color for Hantzsch Method
Acetylacetone (diacetyl acetone) , YELLOW
56
Lipoprotein Analysis : Reference Method and is measure in Svedverg units
Ultracentrifugation
57
Reagent for Ultracentrifugation
Potassium bromide with 1.063 density
58
Ultracentrifugation: Floating Layer
Chylomicron, VLDL
59
Ultracentrifugation: Sinking Layer
LDL, HDL
60
Preferred Gel for Electrophoresis
agarose gel
61
Electrophoresis: from origin to the most anodal
Chylomicron, LDL, VLDL, HDL

CC1 MIDTERM - LIPIDS (1 decks)

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