Liver and Renal

Question 1

Liver Stains

Answer 1

Trichrome - Collagen 
Retic - Architecture of hepatic plate
Pearl's - Iron 
PAS - Glycogen 
Congo Red - Amyloid 
Rhodanine - Copper 
ZN - Fast bacteria
Gram - Bacteria
Grocott - Fungus

Question 2

Liver Trichrome

Answer 2

stains type 1 collagen that is normally present in the portal tracts and vessel walls, but
also highlights the presence and distribution of reactive fibrosis as a result of liver injury. It is used for staging of
chronic liver diseases, and helps to delineate patterns of
injury, such as the perisinusoidal fibrosis associated with
steatohepatitis and periductal fibrosis in primary sclerosing
cholangitis

Question 3

Liver Reticulin

Answer 3

By highlighting these fibers, the stain helps in the assessment of the architecture of the hepatic
plates, such as expansion in regenerative and neoplastic conditions, compression of plates in nodular regenerative hyperplasia,4 and collapse of the reticulin framework in necrosis

Question 4

Liver Iron

Answer 4

In hemochromatosis, iron accumulates primarily in the cytoplasm and initially in the periportal hepatocytes. In secondary iron overload, the accumulation is mainly in the Kupffer cells.

Question 5

Rhodanine Principle

Answer 5

is used to detect copper-binding protein and is thus used to evaluate for Wilson disease. Copper is excreted in bile and accumulates in the liver in chronic biliary diseases

Question 6

Rhodanine Method

Answer 6

1. Bring sections to distilled water
2. Stain in DMAB-rhodanine stain at 60°C 3 hrs (Check microscopically after 1 hour).
3. Rinse well in distilled water
4. Lightly counterstain with alum haematoxylin 5 secs
5. Differentiate in acid alcohol
6. Wash in water
7. Blue in Scotts
8. Wash in water 5 mins
9. Dehydrate, clear and mount

Question 7

Rhodanine Results

Answer 7

copper deposits…………………………brick red

    nuclei. .........................................pale blue
    bile. ...........................................green

Question 8

Renal Stains

Answer 8

Trichrome, congo red, grocott, PAS, EVG, MSB

Question 9

Renal Trichrome

Answer 9

assesment of interstitial fibrosis

Question 10

Renal Grocott

Answer 10

assessment of glomerular basement membrane

Question 11

Renal Congo

Answer 11

demonstration of amyloid

Question 12

Renal PAS

Answer 12

demonstration of arteriolar hyalinosis, and

basement membranes

Question 13

Renal EVG

Answer 13

1. Bring sections to distilled water
2. Oxidise with potassium permanganate 5 mins
3. Rinse with distilled water
4. Decolourise with oxalic acid 1 mins
5. Wash with distilled water
6. Rinse in 95% alcohol
7. Stain in Miller’s stain 1½ - 3hrs
8. Wash in 95% alcohol
9. Rinse with distilled water
10. Stain nuclei with Celestin Blue 5 mins
11. Rinse in distilled water
12. Stain in Haematoxylin 5 mins
13. Wash in running tap water 5 mins
14. Rinse with distilled water
15. Counterstain with Curtis’ stain 2 mins
16. Blot dry
17. Rapidly dehydrate, clear and mount.

Question 14

Renal EVG Results

Answer 14

elastic fibres, Mast cell granules…………………jet black

      collagen.................................................red

      muscle, cytoplasm, fibrin.............................yellow

      nuclei. ...................................................brownish green
      erythrocytes. ...........................................yellow