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Liver Function Test Flashcards

1
Q

Perform majority function of liver;
Degenerative property of liver

A

Hepatocyte

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2
Q

Act as phagocyte

A

Kupffer cells

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3
Q

Line the sinusoid of the liver

A

Kupffer cells

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4
Q

Large organ divided unequally into 2 lobules by

A

Falciform ligament

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5
Q

Functional unit of the liver

A

Lobules

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6
Q

Chief metabolic organization

A

Liver

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7
Q

Responsible for all metabolic and excretory function of liver

A

Lobules

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8
Q

Amount of blood supply in the body

A

1500 ml/minute

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9
Q

Function of liver

A
  1. Synthetic function
  2. Conjugate function
  3. Detoxification and drug metabolism
  4. Excretory and secretory function
  5. Storage function
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10
Q

Ability to synthesize substance (protein)

A

Synthetic function

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11
Q

Metabolism of bilirubin

A

Conjugation function

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12
Q

Bile (elimination of cholesterol and bile salts)

A

Excretory and secretory function

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13
Q

Fat soluble

A

Vitamin A, D, E, K

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14
Q

Albumin + Globulin

A

Total protein

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15
Q

Sample used in total protein

A

Serum

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16
Q

Interference in total protein

A

Hemolysis and ictericia

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17
Q

Reference value for Total protein

A

6.5 - 8.3

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18
Q

Assessment of nutritional status and presence of diagnosis of disease involving kidney, liver and bone marrow

A

Total protein

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19
Q

Methods in total protein

A
  1. Kjeldahl
  2. Biuret
  3. Folin - Ciocalteu (lowry)
  4. UV absorption
  5. Refractometry
  6. Turbidimetry and Nephelometry
  7. Commasie brilliant blue dye
  8. Ninhydrin
  9. Serum protein electrophoresis
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20
Q

Classical method; reference but not routinely used

A

Kjeldahl

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21
Q

Colorimetric non enzyme method

A

Biuret

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22
Q

3 steps in kjeldahl method

A

Digestion
Distillation
Titration

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23
Q

Oxidation of phenolic compounds such as ___

A

Tyrosine, tryptophan & histidine

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24
Q

Principle: oxidation of phenolic compounds

A

Folin - ciocalteu (lowry) method

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25
Based on refractive index of serum
Refractometry
26
Absorbance used in UV absorption method
210 nm
27
Absorption @ 280nm
Tryptophan, tyrosine & phenylalanine
28
Measures the amount of nitrogen
Kjeldahl
29
1g of nitrogen =
6.54 g of protein
30
Uniform fine precipitate which scatter lights or block of light
Nephelometry and turbidity
31
Detection of protein as little as 1 ug; disbonding technique
Coomasie brilliant blue dye
32
Color formed in biuret method
Deep purple / violet
33
Most widely used method for total protein
Biuret method
34
Biuret method absorbance
540 nm
35
Based on peptide bond present in protein with cupric ions
Biuret
36
Interference in biuret method
Lipemic
37
Reagents used in biuret method
Alkaline copper sulfite Sodium potassium tartrate (rochelle salts) NaOH KI (stabilizer)
38
Color formed in Folin- ciocalteu
Deep blue
39
High analytical sensitivity
Folin-ciocalteu
40
Main reagent in folin
Phosphotungstic - molybdic acid/ phenol reagent
41
Folin color enhancer
Biuret reagent
42
Violet color reacting with primary amines widely used for detecting peptides and amino acids after paper chromatography
Ninhydrin
43
Either positive or negative
Amphoteric
44
Migration of charged particles in an electric field
SPE
45
SPE buffer
Barbital (veronal)
46
pH of buffer
6.8 (basic)
47
Reference value of albumin bond
3.5-5.0 g/dl (56-65%)
48
Reference value of a1 globulin
0.1-0.3 g/dl (2.5-5%)
49
Reference value of a2 globulin
0.6-1.0 g/dl (7-13%)
50
Reference value of beta globulin
0.7 - 1.1 g/dl (8-14%)
51
Gamma globulin reference value
0.8-1.6 g/dl (12-22%)
52
The concentration is directly proportional to the severity of hepatic disease
Albumin
53
Methods in albumin
Salt precipitation Dye binding
54
Albumin is precipitated?
No. Globulin is precipitated
55
Albumin is quantitated using biuret reaction?
Yes
56
Binds to positive charge
Anionic dye
57
Dye commonly used
Bromocresol green
58
Most specific dye
Bromocresol purple
59
Not specific to albumin
Methyl orange
60
More specific to albumin
HABA - 2,4 - Hydroxyazobenzene - benzoic acid
61
Interference in dye binding
Conjugated bilirubin Aspirin Sulfonamides
62
Reference value of A/G ratio
1.3-3.1 g/dl
63
Formula of AG ratio
AG ratio = albumin / globulin Globulin = total protein - albumin
64
Vitamin k response test
Prothrombin time
65
Amount administered in PT
10 mg for 1-3 days
66
It differentiate intrahepatic from extra hepatic disorder
PT
67
Prolonged PT
Intra hepatic
68
Not prolong PT
Extra hepatic

CC1 (3 decks)

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