long question (4) Flashcards
1
Q
you are working in a lab investigating fibrosis. you have samples from normal and fibrotic tissue. describe how you would use these samples to identify genes that might be relevant to fibrosis. your lab has a fibrosis cell line. describe experiments that would demonstrate the genes you identified are functionally relevant
A
2
Q
how would you use these samples to identify gene that may be relevant to fibrosis?
A
- check protein expression (western blotting)
- ELISA
- fluorescence
- compare normal and fibrotic tissue (gene expression)
3
Q
describe experiments that would demonstrate genes to identify are functionally relevant
A
- siRNA
- CRISPR