Long Quiz Flashcards by Joo Ary

Formaldehyde is formed from oxidation of: *

a. Ethanol
b. Methanol
c. Isopropanol
d. Propanol
e. Methyl salicylate

b. Methanol

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This metallic fixative is generally indicated for preserving * acid mucopolysaccharides
a. Lead fixative
b. Mercury fixative
c. Chromate fixative
d. Any of the above

a. Lead fixative

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All mercuric chloride containing fixative may produce characteristic black granular deposits except:

a. B-5
b. Heidenhain’s susa
c. Helly’s solution
d. Zenker’s fluid

b. Heidenhain’s susa

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Paraffin wax substitute recommended for embedding the eyes: *

a. Dry celloidin
b. Wet celloidin.
c. Bioloid
d. Tissue mat

c. Bioloid

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Which of the following fixatives may also act as a dehydrating agent? *

a. Formalin
b. Glutaraldehyde
c. Ethanol
d. Orth’s fluid

c. Ethanol

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Autopsy materials should be preserved as soon after death as possible. If this cannot be done, the body should be placed in a refrigerator kept at:

a. 4C
b. 5C
c. 1C
d. 10 C

a. 4C

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Masson-Fontana method is a stain for: *

a. Phospholipid
b. Connective tissue
c. Melanin
d. Tissue and bone

c. Melanin

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Excessive exposure to benzene may damage the bone marrow resulting in: *

a. Iron deficiency anemia
b. Thalassemia
c. Hemoglobinopathies
d. Aplastic anemia

d. Aplastic anemia

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The best decalcifying agent is: *

a. 10% nitric acid
b. HCI
C. 5% formic acid
d. Von ebner’s fluid

C. 5% formic acid

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Saccomano’s fixative is composed of: *

a. Ethanol and carbowax
b. Carbowax and methanol
c. Gelatine and methanol
d. 3-APES

a. Ethanol and carbowax

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Deparaffinization is done using:*

a 95% alcohol
b. 70% alcohol
c. Xylene
d. Acetone

c. Xylene

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What are the method of embedding: *

a Celloidin embedding
b. Paraffin wax embedding
c. Both
d. Neither

c. Both

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In Papanicolaou’s stain, this reagent is not included: *

a. Hematoxylin
b. OG6
c. Eosin
d. EA 36

c. Eosin

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What does “CA” mean in CA-125 *

a. Collagenous antigen
b. Cercarial antigen
c. Cancer antigen
d. Chromic acid

c. Cancer antigen

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Stain used for elastic fibers *

a. Gram’s
b. Ziehl-Neelsen
c. Gomori’s aldehyde-fuschin
d. Hematoxylin agent

d. Hematoxylin agent

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One of the following is not used as an adhesing agent: *

a. Egg albumin
b. Starch
c. Nomal saline
d. Plasma

c. Nomal saline

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Best agent for dehydration is: *

a Benzene
b. 10% formalin
c. Graded Ethyl alcohol
d. Xylene

c. Graded Ethyl alcohol

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All of the following are embedding media except: *

a. Paraffin
b. Celloidin
c. Plastic
d. Cellosolve

d. Cellosolve

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The method of choice for staining is exfoliative cytology is: *

a. Papanicolaou staining
b. Wright staining
c. Hematoxylin and eosin
d. Giemsa staining

a. Papanicolaou staining

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Which of the following stain is used to demonstrate calcium? *

a. Congo stain
b. Lindquist method
c. Von kossa stain
d. Prussian blue

c. Von kossa stain

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The elastic fiber in orcein staining method are colored: *

a. Blue
b. Red
c. Brown
d. Colorless

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c. Brown

Laxol fast blue is special stain for: *

a. Carbohydrates
b. Central nervous system
c. Epithelial basement membrane
d. Fungi

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b. Central nervous system

Which of the following is not a metachromatic dye? *

a. Safranin
b. Thionine
c. Eosin
d. Methyl violet

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c. Eosin

Chromate fixatives: 1. Regaud’s fluid2. Orth’s fluid 3. Moeller’s fluid 4. B-5 *

a. 1,2,3,4
b. 1,2,3
c. 1,2,4
d. 1,3,4

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b. 1,2,3

25. Mercuric chloride fixatives: 1. Zanker's fluid 2. Heidenhain's Susa solution 3. Hell's solution 4. B-5 a. 1,2,3,4 b. 1,2,3 c. 1, 2, 4 d. 1,3.4

a. 1,2,3,4

26. Picric acid fixatives, except: 1. Helly's 2. Heidenhain's Susa 3. Orth's fluid 4. B-5* a. 1. 2. 3. 4 b.1,2.3 c. 4 only d. None.

d. None.

27. Alcohol fixatives: 1. Carnoy's 2. Gendre's 3. Newcomer's 4. Flemming's* a. 1,2,3,4 b. 1,2,3 c. 1,2 d 1,3

d 1,3

28. Osmium tetroxide fixative: 1. Helly's 2. Heidenhain Susa 3. Zenker's 4. Flemming's a. 1, 2, 3, 4 b.2,3,4 c. 2,3 d. 4 only

d. 4 only

29. Recommended size of tissues in order to maintain adequate fixation: * a. 1-3 mm2 b. 2-4 mm2 c. 3-5 mm2 d. 4-6 mm2

c. 3-5 mm2

30. During dehydration, the amount in each stage should not be less than the tissue: a. 5 b. 10 c 15 d. 20

b. 10

31. Most abundant type of cartilage in the adult: * a. Hyaline b. Elastic c.Fibrous d. All are equal

a. Hyaline

32. A good decalcifying agent * a. 1% Glacial acetic acid b. 5% Nitric acid c. 10% Sodium Hydroxide d. Acid Alcohol

b. 5% Nitric acid

33. In Pap's smear, a suggestive but not conclusive diagnosis of malignancy belong to: a. Class I b. Class Il c. Class III d. Class IV

c. Class III

34. Most common fixative for Pap's smear: * a. 75% alcohol b. 85% alcohol c. 95% ethyl alcohol d. 95% methvl alcohol

c. 95% ethyl alcohol

35. Best clearing agent: * a. Acetone b. Xylene c. Formalin d. Methanol

b. Xylene

36. Special stain for glycogen: * a. Sudan stain b. Wright's stain c. Fontana stain d. Periodic acid Schiff solution

d. Periodic acid Schiff solution

37. The process in the preparation of microscopic slide that follows clearing is: * a. Impregnation b. Dehydration c. Fixation d. Embedding

a. Impregnation

38. An excellent fixative for electron microscopy is: * a. Glutaraldehyde b. Formaldehyde c. Acetone d. Alcohol

a. Glutaraldehyde

39. The optimum temperature in degrees centigrade of the water bath in tissue processing a. 35-38 b. 20-30 c. 46-50 d. 40-45

c. 46-50

40. Most waxes for embedding in histologic sectioning melt at this degrees centigrade a. 37 b. 45 c. 30 d. 56

d. 56

41. The ascending grade of alcohol in H&E staining method is used for: * a. Hydration b. Clearing c. Dehydration d. Fixation

c. Dehydration

42. Trichrome stain is a special stain for: * O a. Fat b. Amyloid c. Collagen d. Mucin

c. Collagen

43. What is the concentration of formalin as a fixative: * a. 5% b. 10% c. 15% d. 40%

b. 10%

44. Embedding is also: * a. Clumping b. Infiltration c. Casting d. Clearing

c. Casting

45. An ideal fixative should be * a. Cheap and safe to handle b. Cheap, stable, and safe to handle c. Cheap and stable d. Stable and safe to handle

b. Cheap, stable, and safe to handle

46. Mucicarmine stain is for staining: * a. Mucin b. Celloidin c. Amyloid d. Iron

a. Mucin

47. Silver impregnation method of staining is used to demonstrate * a. Glycogen b. Spirochetes c. Hemosiderin d. Polysaccharide

b. Spirochetes

48. Resealing the paraffin blocks with melted paraffin will: * a. Harden tissue b. Intensify nucleus c. Melt block d. Prevent drying

d. Prevent drying

49. This solution can be used instead of xylol: * a. Chloroform b. Ethyl alcohol c. Acetone d. Isopropyl alcohol

a. Chloroform

50. Trimming is done until block forms a: * a. Triangular surface b. Rectangular surface c. Flat surface d. 4-sided prism

d. 4-sided prism

Long Quiz Flashcards

(50 cards)