MASS SPEC Flashcards
(33 cards)
An instrumentaltechnique used to confirm the molar mass of a substance or molecule through the use of a mass spectrometer
mass spectrometry
instrumental analytical technique used to measure the mass-to-charge ratio of fragment ions in order to elucidate the chemical identity and structure of molecules.
mass spectrometry
bombards the molecule under investigation with an electron beam and quantitatively records the result as a spectrum of positive ion fragments (mass spectrum)
mass spectrometer
It is the transition of the analyte from the solid or liquid phase into the vapor
phase.
Volatilization
the first crucial and challenging step in the
analysis of organic compound through mass spectrometry.
Ionization
This involves the conversion of neutral molecule to a gas-phase ionic specie with the use of various techniques such as ejection or capture of an electron by an
analyte.
Ionization
move randomly and their separation by gravitational force seems highly impractical
neutral gas-phase species
the component of the mass spectrometer that takes ionized fragments and separates them based on charge to mass ratios and outputs them to the detector where they are detected and later converted into a digital output.
mass analyzer
are used for mass focusing and focus angular dispersions. It separates the ions while on flight using a magnetic field. (electrostatic field before magnetic field)
Magnetic field deflection spectrometer
separates ions of different masses by
making use of their different velocities after
acceleration through a potential V
Time of flight spectrometer
It separates ions by time without the use of electric or magnetic field. The separation of ions is based on the kinetic energy and velocity of the ions in similar sense as that of chromatography.
Time of flight spectrometer
It uses an electric field for the separation of ionic fragments by mass to charge ratios.
Quadrupole spectrometer
It uses a magnetic field to trap ions into an orbit inside of it.
Ion cyclotron resonance mass spectrometer
there is no occurrence of ion separation rather all the ions of particular range of m/e values are trapped inside and an applied external electric field helps to generate a signal.
Ion cyclotron resonance mass spectrometer
This utilizes two or more spectrometers which are coupled together using an additional reaction step to increase their abilities in analyzing chemical samples.
Tandem mass spectrometer
The ion is vibrationally excited by collision with the target gas (i.e. Argon) that generates ion fragments that are separated and recorded by a second mass analyzer
Collision-induced dissociation (CID)
It is a method of fragmenting gas phase ions for structural elucidation
of peptides and proteins.
Electron capture dissociation (ECD)
It is a method of fragmenting multiply-charged gaseous macromolecules in a mass spectrometer between the stages of
tandem mass spectrometry.
Electron transfer dissociation (ETD)
It is a technique used in mass spectrometry to fragment molecules in the gas phase for structural analysis of the parent molecule.
Infrared multiproton dissociation (IRMPD)
Ions are trapped in an ultrahigh vacuum by a magnetic and an electric field and rapidly exchanged blackbody photons with the vacuum chamber walls in the mass spectrometer.
Blackbody infrared radiative dissociation (BIRD)
device for introducing the sample
Sample handling system
for determining the
amount of sample introduced
micromanometer
a device for metering the sample to the ionization chamber
molecular leak
This component is responsible for the fragmentation of molecules before they
are mass analyzed.
Ionization & accelerating chambers
