Mass spectrometry- PAUL

Question 1

What is mass spec?

Answer 1

Tool used for measuring the mass to charge ratio of one or more molecules present in sample (m/z)

Question 2

How many main steps are there?

Answer 2

1. Ionisation
2. Mass analysis
3. Detection
4. Mass spec

Question 3

What can mass spec help determine?

Answer 3

Isotopes - e.g., chlorine 35 and 37 -35 more abundant

Question 4

Mass^n

Answer 4

Fragment the molecule into atoms
-always one fragment more stable than the rest, tallest peak

Question 5

What peak suggests one fragment is more stable than the rest?

Answer 5

Tallest peak on mass spec graph

Question 6

Where does fragmentation happen?

Answer 6

Ionisation chain