Mass Transport Flashcards
(92 cards)
What type of molecule is hemoglobin?
Protein
How many polypeptide chains make up a haemoglobin molecule?
Four
What does haemoglobin transport?
Oxygen
What is the primary structure of haemoglobin?
Order of amino acid sequence in polypeptide chains
What is the secondary structure of haemoglobin?
Coiling of polypeptide chains into a helix
What is the tertiary structure of haemoglobin?
Folding polypeptide chains into precise shape.
What is the quaternary structure of haemoglobin?
Linking of the four polypeptide chains. Each polypeptide chain also also has an associated haem group.
What ion does haem group contain?
Iron (Fe2+)
How many oxygen molecules does each iron ion combine with?
one per ion
How many oxygen molecules can a molecule of haemoglobin carry in humans?
four
What is the name given to the process by which haemoglobin binds to oxygen?
Loading
Association
What is the name given to the process by which haemoglobin releases oxygen?
Unloading
Disassociation
What does affinity mean with respect to haemoglobin?
Affinity is the relative attraction of haemoglobin to oxygen
If haemoglobin has high affinity, what does this mean?
Easier to take in oxygen, harder to lose
If haemoglobin has low affinity, what does this mean?
Harder to take in, easier to lose
How does haemoglobin change its affinity for oxygen?
It will change shape when in the presence of certain molecules (e.g. carbon dioxide). If present, the carbon dioxide changes its shape, so the oxygen no longer fits as well, and is easily disassociated.
What do we mean by partial pressure?
Pressure exerted by a single component of a mixture of gases
What does a dissociation curve represent?
Relation between partial pressure of oxygen and oxygen saturation of haemoglobin
What shape does the oxygen dissociation curve take?
Sigmoidal (S)
Why does the oxygen dissociation curve increase slowly to begin with>
Difficult for first oxygen molecule to bind
Why does the oxygen dissociation curve increase sharply after a slow beginning?
First oxygen to bind changes the quarternary structure, making the binding of subsequent molecules easier
Why does the oxygen dissociation curve increase slowly at the end of the graph?
Easier for molecules to bind, but the likelihood of the fourth oxygen molecule finding an empty binding site is less likely.
What is the name of the increase in ease of binding for oxygen molecules?
Positive cooperativity
If an oxygen dissociation curve is shifted to the left, how does that affect the affinity for oxygen?
Increases oxygen affinity