MEASLES

Question 1

what are the characteristics of the family Paramyxoviridae

Answer 1

• spherical
• pleomorphic
• single-stranded
• nonsegmented
• negative sense
• not segemented genome

Question 2

what are the three (3) proteins complexed with Viral RNA

Answer 2

1. nucleoprotein
   2-3 P and L

Question 3

Composition of Paramyxoviridae

Answer 3

Protein (73%)
lipid (20%)
carbohydrate (6%)
RNA (1%)

Question 4

3 proteins that participate in the formation of viral envelope:

Answer 4

1. matrix (M) protein
2. larger glycoprotein (HN or G)
3. glycoprotein F

Question 5

it mediates membrane fusion and hemolysin activities

Answer 5

Glycoprotein F

Question 6

it represent the major internal protein and it forms the helical capsid

Answer 6

Nucleocapsid (N)

Question 7

it is important in virion assembly and underlies the viral envelope

-essential for particle formation

Answer 7

matrix (M) protein

Question 8

involved in the viral polymerase activity and function as transcription and RNA replication

Answer 8

P and L

Question 9

responsible for attachment to the host cell

Answer 9

larger glycoprotein (HN or G)

Question 10

TRUE OR FALSE

virions infectivity is lost readily when the envelope is disrupted spontaneously and when the virus is treated with lipid solvents

Answer 10

TRUE

virions infectivity is lost readily when the envelope is disrupted spontaneously and when the virus is treated with lipid solvents

Question 11

What is the receptor of paramyxovirus

Answer 11

CD46 and CD150 molecule

Question 12

Paramyxoviruses attach to the host cells via what?

Answer 12

hemagglutinin glycoprotein (HN,H or G protein)

Question 13

it undergoes complex refolding during the process of viral and cellular membrane fusion

Answer 13

F1 protein

Question 14

TRUE or FALSE
the most abundant class of transcription produced by an infected cell is form the L gene, located at the 5’ end , the least abundant is the N gene, located at the 3’ end

Answer 14

FALSE

the most abundant class of transcription produced by an infected cell is from the N gene, located at the 3’ end , the least abundant is the L gene, located at the 5’ end

Question 14

TRUE or FALSE
the most abundant class of transcription produced by an infected cell is form the L gene, located at the 5’ end , the least abundant is the N gene, located at the 3’ end

Answer 14

FALSE

the most abundant class of transcription produced by an infected cell is from the N gene, located at the 3’ end , the least abundant is the L gene, located at the 5’ end

Question 14

TRUE or FALSE
the most abundant class of transcription produced by an infected cell is form the L gene, located at the 5’ end , the least abundant is the N gene, located at the 3’ end

Answer 14

FALSE

the most abundant class of transcription produced by an infected cell is from the N gene, located at the 3’ end , the least abundant is the L gene, located at the 5’ end

Question 15

viral protein are synthesized in the?

Answer 15

cytoplasm

Question 16

viral protein are synthesized and glycosylated in the?

Answer 16

secretory pathway

Question 17

what is the order of genes in terms of their products

Answer 17

N, P,M, F,H, and L

Question 18

how does paramyxovirus mature

Answer 18

through BUDDING

Question 19

it lacks neuraminidase but has hemagglutinating activity

Answer 19

measles virus/rubeola virus

Question 20

what is the natural host of the measles virus

Answer 20

humans

other host include: monkey, dogs, mice

Question 21

primary site of infection in humans

Answer 21

respiratory epithelium of the nasopharynx
(CDC) alveolar macrophages or dendritic cells