Measuring proteins and enzymes Flashcards
(7 cards)
1
Q
how can protein abundance be measured via spectophotometry
A
mixing the proein with a dye
2
Q
at what level can prtoein be detected
A
595nm
3
Q
at what wavelength does nucleic acid absorb uv light
A
260nm
4
Q
what is the western blot
A
most widely used lab technique to detect specific protein in a tissue homogenate or protein extract
5
Q
what is process of QRT-PCR
A
sample collection - RNA extraction - Reverse transcription - RT-PCR amplification - Results
6
Q
what technique is best suitede to measure total protein concentration
A
bradford essay
7
Q
A
