Mechanisms Of Inheritance & Biotech. Flashcards

(57 cards)

1
Q

What is DNA

A

Deoxyribonucleic acid (a polymer). DNA is the biological molecule of inheritance.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What shape is DNA in?

A

A double helix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is DNA made up of?

A

Sugars - sides
Phosphate - sides
Bases (nitrogens) - rung

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are the 4 bases

A

Guanine
Cytosine
Thymine
Adenine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Which bases are purines and which are pyrimidines

A

Adenine & Guanine are purines (2 rings)

Thymine and Cytosine are pyrimidines (1 ring)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How are nucleotides connected to one another?

A

By covalent bonds between phosphate and sugar.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is DNA replication

A

The copying of DNA before mitosis and meiosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Where does DNA replication occur

A

In the nucleus during interphase just before prophase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What are the 4 enzymes that help DNA replication occur

A

Helical, DNA Polymerase, Primase, and Ligase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What does Helicase do

A

Unwinds and unzips by breaking the hydrogen bonds between bases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What does DNA Polymerase do

A

Builds the new DNA strand

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What does the Primase do

A

initializes the entire process and sets a path for POLYMERASE to follow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What does Ligase do?

A

Glues the Okazaki fragments on the lagging strand

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is unzipping?

A

When the Helicase breaks the hydrogen bond forming a replication fork

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is the first step of DNA Replication

A

The origin is discovered and the strand is unwound (by the helicase) forming a replication fork

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is step two in DNA Replication

A

SSB (single stranded binding) proteins bind to each of the DNA sides to keep it unwound

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What is step 3 in DNA Replication

A

Primase forms a primer on both sides of the unzipped parent DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is the 4th step in DNA Replication

A

DNA Polymerase recognize the primer and uses free nucleotides t build the DNA strand by using the parent code

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What is the 5th step of DNA Replication

A

Covalent bonds are formed between phosphates and sugars

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Note: DNA is anti parallel, meaning……

A

Each strand is built differently. One strand is leading and one is lagging, therefore it is built in small fragments.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Which strand of DNA is leading and which strand is lagging

A

The strand with the number of carbons going from 5’ to 3’ is lagging, and the one going from 3’to 5’ is leading

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What is the 6th step of DNA Replication

A

DNA polymerase reads the DNA 3’ to 5’ (building 5’ to 3’)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What is the 7th and final step of DNA Replication

A

Ligase joins the Okazaki Fragments on the lagging side (5 to 3)

24
Q

What is RNA

A

It stands for Ribonucleic Acid, and is a bio molecule made of ribonucleotides

25
DNA vs. RNA
DNA RNA ——————————-)——————————- - Deoxyribonucleic acid ) - Ribonucleic acid - Codes for traits. ) - used in protein synthesis - Double stranded. ) - single stranded (twisted & coiled). ) - Found in nucleus ) - Found in nucleus & cytoplasm - sugar is deoxyribose ) - sugar is ribose - one type. ) - three types
26
What bases is RNA made of
Adenine Uracil Guanine Cytosine
27
What are the 3 types of RNA
``` Messenger RNA (mRNA) which carries the instructions from the nucleus to the cytoplasm Ribosomal RNA (rRNA) and transfer RNA (tRNA) which are both involved in the process of ordering the amino acids to make the protein. ```
28
What is the 1st step in transcription
DNA is transcribed into a small message to be carried to a ribosome (by a mRNA)
29
What is the 2nd step in transcription
RNA polymerase ( an enzyme ) is used to “read” the message by “matching” the DNA code w. RNA bases
30
What is the 3rd & final step in transcription
The mRNA is transported out of the nucleus, to find a ribosome in the cytoplasm
31
What is the 1st step in translation
In the cytoplasm, mRNA moves to a ribosome made of rRNA where the protein is assembled
32
What is the 2nd step in translation
tRNA carries amino acids according to its anticodon, while mRNA holds the codons in the cytoplasm
33
What are chromosomal mutations
Chromosomal mutations involve changes in the number or structure of chromosomes
34
What are the 4 types of Chromosomal mutations
1) Deletion 2) Duplication 3) Inversion 4) Translocation
35
What is deletion
A mutation that involves the loss of all of part of a chromosome
36
What is Inversion
A mutation that reverses the direction of parts of a chromosome
37
What is duplication
A mutation that produces an extra copy f all or part of a chromosome
38
What is translocation
A mutation where one part of a chromosome breaks off and attaches to another
39
What are gene mutations
Mutations that involve changes n one or a few nucleotides
40
What are the 3 types of gene mutations
1) Substitution 2) Deletion 3) Insertion
41
What is substitution
A mutation where a single nucleotide is replaced or substituted
42
What is Deletion
A mutation similar to insertion, it shifts the reading frame of a genetic message and can change all of the amino acids after that mutation
43
What is insertion
A mutation where a single nucleotide is inserted into the DNA sequence causing a frameshift to occur, leading to all the amino acids after to be changed
44
What is recombinant DNA
DNA produced by combining DNA from different sources
45
What is gene therapy
When cloned genes are used to modify a human
46
What is a clone
An organism with genetically identical cells produced from a single cell
47
How do you create a clone
You take and grow a single cell that is genetically identical to the adult organism until it becomes an entirely new individual that is genetically identical to the owner of then original cell
48
Why are identical twins considered clones
The 1st and 2nd cells of the embryo separated and each became a complete individual
49
What are 3 reasons that people clone genes
1) To produce a large quantity of the genes protein product ex//insulin 2) To learn how a cloned gene codes for a particular protein 3) To use the genes to alter the phenotypes of other organisms in a beneficial way
50
What is a transgenic organism
An organism that contains genes from other organisms
51
How are transgenic organisms created
By inserting recombinant DNA into the genome of a host organism
52
What did franklin do
She produced the X-ray crystallography pictures of DNA
53
What did Fredrick Meischer do
He isolated the chemicals that made up white blood cells, which he called nuclien
54
What did McClintock do
She showed that chromosomal crossovers happen in corn chromosomes, and later discovered that x-rays could cause translocations, inversions, deletions, and ring chromosomes in corn
55
What did Avery do
He discovered transformation in bacteria, and proved cell fragments of dead, disease-causing bacteria could still cause pneumonia when allowed to come in contact with harmless bacteria
56
What did Hershey and Chase do
They studied bacrophages (a type of virus that attacks bacteria) and discovered that viral DNA had the info to produce more viruses
57
What did Crick do
Correctly proposed the structure of DNA, as well as ran the Human Genome Project from 1988 to 1992, and made the cold spring harbor lab