Medical Microbiology Lab 1- POD242 Flashcards
(18 cards)
What is the importance of proper specimen collection?
Ensures accurate diagnostic results
Proper specimen collection is critical for reliable laboratory findings.
What methods are used for culturing and isolating microorganisms?
Microscopy, culture, biochemical testing, serology testing, molecular testing
These methods are essential for identifying microbial agents.
What are key techniques for microbial identification?
Gram staining
Gram staining is a fundamental technique for differentiating bacteria.
What is the clinical relevance of lab findings in diagnostics?
Helps in identifying pathogens and guiding treatment decisions
Accurate lab work bridges diagnostics with clinical care.
What are common infection control practices in microbiology labs?
Antimicrobial stewardship, safety measures, and proper handling of specimens
Infection control is critical to prevent contamination and ensure safety.
What does MIC stand for in sensitivity testing?
Minimum Inhibitory Concentration
MIC is a key measurement in assessing antibiotic effectiveness.
What are the types of growth media?
Enriched media, selective media, differential media, simple media
Each type serves a specific purpose in microbial growth.
What is enriched media used for?
To allow the widest variety of microorganisms to grow
Enriched media provide extra nutritional elements for fastidious organisms.
What is selective media designed to do?
Allow only certain microorganisms to grow
Selective media helps in isolating specific types of bacteria.
What is differential media used for?
To help identify microorganisms by differentiating between them
Differential media can indicate metabolic properties of organisms.
Fill in the blank: Microorganisms grow best in a _______ environment.
warm, dark, moist, protein-rich
These conditions mimic the natural habitats of many microbes.
What physical conditions are provided for microbial growth during incubation?
Optimum temperature, oxygen, light/darkness
Incubation conditions are vital for successful microbial growth.
What is the purpose of macroscopic examination in microbiology?
To observe growth characteristics of microbial colonies
Macroscopic examination helps in preliminary identification.
What are the steps involved in Gram’s staining?
Smear preparation, fixation, primary stain, mordant, decolorization, secondary stain, rinsing, visualization
Each step is crucial for accurate Gram classification.
True or False: Gram-positive bacteria stain purple.
True
Gram-positive bacteria retain the crystal violet stain.
What causes bacteria to stain as Gram-positive or Gram-negative?
Differences in cell wall components
The structure of the cell wall affects stain retention.
What examples of bacteria can be identified using Gram staining?
Streptococcus pyogenes, Escherichia coli
These are common examples used in microbiology labs.
What is the role of medical microbiology labs?
Identifying pathogens
These labs are vital for diagnosing infections and guiding treatment.
