mem Flashcards
(22 cards)
initiation of DNA synthesis by DNA polymerase requires
a primer of DNA and RNA
What properties does the mini prep method use
solubility
negative charge
denaturation/renaturation
Nitrogenous bases absorb maximally at
260nm
Omitting centrifugation after precipitation salts added would result in
contamination by genomic dna
Blue white selection refers to
inactivating the beta galactosidase enzyme
the lagging strand is the one that is
looped by the DNA polymerase
Microarray can be used for
gene expression profiling
comparative genome screening
allelic variation screening
expression of many genes at one time
we use cDNA in a microarray because
it is more stable
we use reverse transcriptase, RNase H and DNA polymerase to make cDNA
Microarrays measure
the quantity of mRNA in the form of cDNA
Transfection is
the introduction of foreign DNA into eukaryote cells.
could be done by a gene gun, electroporation or modification of membrane channels
A 2’ hydroxyl group is required for
rna splicing to occur
original allele in HR is eliminated by
DNA repair enzymes
In a PCR, low MgCl2
reduced polymerase activity
Phosphate on:
ser, thr, tyr
drives assembly of protein into larger complexes
Acetyl on lys:
helps activate genes in chromatin by modifying histones
Ubiquitin on lys:
a polyubiquitin chain targets a protein for degradation
telomerase most active in
stem cells and gametes
a transposon is
a mobile section of chromosomal DNA that is allowed to change position on loci of chromosomes.
They can change in 1, between chromosomes and between cells/species
DOES THE MOLECULE STOP TRANSCRIPTION?
YES = REPRESSIBLE NO = INDUCIBLE
DOES THE MOLECULE BINDING TO A PROTEIN STOP OR START TRANSCRIPTION
STOP = -ve regulation START = +ve regulation
Heterozygote advantage to a disease which are symptomatic are:
heterozygous recessive
gene families evolve as
heteroduplexes as a result of recombinant DNA chromosomes
