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M2M - Cell Physiology > Membrane Fusion > Flashcards

Flashcards in Membrane Fusion Deck (2)
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1
Q
  • What is subcellular protein targeting? When this goes wrong? Ex?
  • Basic principles of fusion: Automatically fuse? Must remove what first? What else is important?
  • 3 Snare Proteins:
    1? Structure? Located on?
    2? Structure? Location?
    3? Structure? Location?
  • Binding rule?
  • Same for every pathway? Why?
  • Steps of fusion? (3)
A
  • vessicle transport; incorrect transport; CF
  • No; remove water first; high specificity
    1: VAMP: Transmembrane domain and helical domain in it –> only vessicles
    2: Syntaxin: Transmembrane and helical domain –> membrane
    3: SNAP25: No transmembrane domains; two helical domains –> membrane
  • 3Q:1R
  • No; increase specificity
    1. ) Helical domains bind to make tetramer 2.) squeeze water out 3.) Zipper up and overcome repulsion
2
Q
  • Role of NSF?
  • Role of Sec1?
  • Snare cycle? (4)
    Envelope virus fusion: Same process? How many helices? Ex? (3) Specific?
  • How does influenza do this?
  • How does HIV do this?
A
  • Regulates dissoc. of SNARE after fusion
  • Refolds syntaxin after NSF unwinds it
    1. ) Tetramer formed 2.) Lipid fusion 3.) NSF mediated unwinding 4.) SEC1 refolding
  • No but similar; 4; Ebola, HIV, Influenza; yes
  • Transmembrane domain with hydrophobic fusion domain that refolds with fusion with change in pH
  • Targets CD4 and similar method