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YR2: Musculoskeletal > Metabolic Bone Disease – Biochemistry > Flashcards

Metabolic Bone Disease – Biochemistry Flashcards

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1
Q

What is metabolic bone disease?

A

A group of diseases that cause a change in bone density + strength by increasing bone resorption, decreasing bone formation or altering bone structure

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2
Q

What are the five main metabolic bone disorders?

A 
Primary Hyperparathyroidism 
Osteomalacia/ Rickets  
Osteporosis  
Renal Osteodystrophy 
Paget’s Disease
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3
Q

What are the 4 main components of bone strength?

A

Mass
Material properties (collagen, lamellar, mineralisation)
Microarchitecture (trabecular thickness + connectivity)
Macroarchitectue (Diameter)

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4
Q

When is peak bone mass reached?

A

~25 years

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5
Q

When does bone mass begin to decline?

A

~ 40 years

in women, decline in bone mass accelerates after menopause

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6
Q

How are microfractures repaired?

A

Bone remodelling

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7
Q

Briefly describe the bone remodelling cycle.

A
  1. Microcrack crosses canaliculi + severs osteocyte processes, inducing osteocyte apoptosis
  2. This signals to surface lining cells, which release factors to recruit cells from blood + marrow to the remodelling compartment
  3. Osteoclasts are generated locally + resorb the matrix + the microcrack
  4. Osteoblasts deposit new lamellar bone
  5. Osteoblasts that become trapped in the matrix become osteocytes
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8
Q

What is the normal range for serum total calcium?

A

2.15-2.56 mmol/L

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9
Q

Describe the distribution of calcium.

A

46% PPB (albumin)
47% free calcium
7% complexes (with phosphate or citrate)

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10
Q

What is the ‘corrected’ calcium level?

A

Compensates for changes in protein level (if proteins are high, it compensates down)

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11
Q

Describe the effect of metabolic alkalosis on calcium distribution. What effect does venous stasis have on free calcium levels?

A

Alkalosis: Makes more calcium bind to plasma proteins thus reducing free calcium levels
Venous stasis may elevate free calcium

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12
Q

What are the two main targets of PTH?

A

Kidneys

Bone

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13
Q

Describe the effects of PTH in:

a. Bone
b. Kidneys

A

Bone
Acute release of available calcium (not stored in hydroxyapatite crystal form)
More chronically, increased osteoclast activity
Kidneys
Increased calcium reabsorption at DCT
Increased phosphate excretion at PCT
Increased stimulation of 1-alpha hydroxylase (thus increasing calcitriol production leading to increased gut absorption of Ca)

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14
Q

Where does the PTH-mediated increase in calcium reabsorption take place in the nephron?

A

DISTAL convoluted tubule

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15
Q

Where does the PTH-mediated increase in phosphate excretion take place in the nephron?

A

PROXIMAL convoluted tubule

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16
Q

How many amino acids make up PTH and which part of this is active?

A

84

Active: N1-34

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17
Q

What is PTH dependent on? In what patient group could this cause low PTH?

A

Magnesium

Hypomagnesemia in alcoholics = low PTH + low Ca

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18
Q

What is the half-life of PTH?

A

8 mins

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19
Q

What else can the PTH receptor be activated by other than PTH?

A

PTHrP (PTH related protein)

This is produced by some tumours

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20
Q

What does the parathyroid gland use to monitor serum calcium?

A

Calcium-sensing receptors

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21
Q

Describe the relationship between PTH level and calcium in vivo.

A

Steep inverse sigmoid function

There is a minimum level of PTH release (it can’t get below this even in the case of hypercalcaemia)

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22
Q

What are the causes of primary hyperparathyroidism?

A

Parathyroid adenoma (80%)
Parathyroid hyperplasia (20%)
Parathyroid cancer
Familial syndromes

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23
Q

What 4 biochemical results are diagnostic of primary hyperparathyroidism?

A

HIGH total/ ionised calcium
PTH HIGH or in upper half of normal range (negative feedback should drop PTH if there is hypercalcaemia)
Increased urine calcium excretion
Cr may be high

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24
Q

What are the clinical features of primary hyperparathyroidism?

A

Stones: renal colic, nephrocalcinosis
Bones: osteitis fibrosa cystica
Abdominal moans: dyspepsia, pancreatitis, constipation, anorexia
Psychic groans: depression, impaired concentration
Fractures secondary to bone resorption
Diuresis (polyuria + polydipsia)

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25
What is the main site of action of calcitriol and what effect does it have?
Small intestine: increases calcium + phosphate absorption
26
Describe the effects of calcitriol on bone, kidneys and gut
Facilitates PTH effect on the DCT in the kidneys (increased calcium reabsorption) Synergises with PTH in the bone to increase osteoclast activation Intestines: Activates Calcium + phosphate absorption
27
Which receptors/proteins are involved in mediating the effects of calcitriol on the intestines?
TRPV6 | Calbindin
28
What parameter is used to determine whether a patient is vitamin D deficient?
Deficient < 20 ng/M (50 nmol/L) | Normal > 30 ng/M (75 nmol/L)
29
What is Rickets?
Inadequate vitamin D activity leads to defective mineralisation of the cartilaginous growth plate (before a low calcium)
30
State 8 signs and symptoms of Rickets.
``` Symptoms: Lack of play Bone pain and tenderness (axial) Muscle weakness (proximal) Sign: Age dependent deformity Myopathy Hypotonia Short stature Tenderness on percussion ```
31
State 5 Vitamin D related causes of Rickets/Osteomalacia.
``` Dietary deficiency Malabsorptoin Drugs e.g. enzyme inducers such as phenytoin Chronic renal failure Rare hereditary ```
32
For each of the following state whether it would be high, low or normal in the serum of a Rickets patient: a. Calcium b. Phosphate c. Alkaline Phosphatase d. 25-OH cholecalciferol e. PTH f. URINE phosphate
``` Calcium: Normal or Low Phosphate: Normal or Low Alkaline Phosphatase: High 25-OH cholecalciferol: Low PTH: High (compensatory) URINE phosphate: High ```
33
Other than PTH, what else can cause increased phosphate excretion?
FGF23
34
What effect does FGF23 have that is unlike PTH?
It inhibits 1 alpha-hydroxylase, thus inhibiting calcitriol production
35
Which cells produce this FGF23?
Osteoblast lineage cells
36
Other than Vitamin D deficiency, what else can cause Rickets/Osteomalacia?
Phosphate deficiency
37
State 5 phosphate-related conditions that cause Rickets/Osteomalacia.
X-linked Hypophosphataemic Rickets (mutation in Phex (this cleaves FGF23)) Autosomal Dominant Hypophosphataemia Rickets Oncogenic Osteomalacia (mesenchymal tumours can produce FGF23) PCT damage causes phosphaturia + stops 1a-hydroxylation of Vit D Fanconi syndrome
38
What can cause osteoporosis due to increased bone resorption and decreased bone formation?
Glucocorticoids
39
How does oestrogen deficiency lead to a decrease in bone mineral density?
It increases the no. of bone remodelling units Causes an imbalance in bone remodelling with increased bone resorption compared to bone formation Causes remodelling errors Causes decreased osteocyte sensing
40
Describe the biochemistry of someone with osteoporosis.
Everything should be normal if the cause is primary
41
What is the single best predictor of fracture risk?
BMD
42
What is used to measure BMD?
DEXA scans
43
Which bones are used when measuring BMD and why?
Vertebral bodies Commonest fracture Good measure of cancellous bone It is a highly metabolically active bone so it is quick to respond to treatment Hip: 2nd commonest fracture fracture risk assessment tool (FRAX) uses hip BMD
44
Which chains make up type 1 collagen?
2 x alpha 1 | 1 x alpha 2
45
What can be used as a marker of bone formation that is linked to collagen production?
Procollagen type 1 N-terminal propeptide (P1NP) | an extension peptide that is cut off + can be measured in the blood
46
What can be used as a measure of bone resorption that is linked to collagen production?
C-terminal telopeptide (CTX): serum N-terminal telopeptide (NTX): urine 3 hydroxylysine molecules on adjacent tropocollagen fibrils condense to form a pyridinium ring linkage which can be measured
47
After how long do bone resorption markers fall?
4-6 weeks
48
What are the 4 problems with cross-linking collagen, with regards to measurement of bone markers?
Reproducibility Positive association with age Need to correct for creatinine Diurnal variation in urine markers
49
What bone formation marker is commonly in use?
Alkaline Phosphatase
50
What is Alkaline phosphatase as a bone formation marker used in the diagnosis and monitoring of?
Osteomalacia Paget’s Bone Metastases
51
What is P1NP being used for now?
Used as a predictor of response to anabolic treatments
52
What are the two forms of alkaline phosphatase?
Liver | Bone
53
Which bone diseases will cause a rise in ALP?
Osteomalacia Bone metastases Paget's disease Also hyperparathyroidism and hyperthyroidism
54
How does alkaline phosphatase change with age?
Increases markedly during puberty reaching its highest levels Remains relatively constant following puberty (potential small rise > age 50)
55
What biochemical changes occur in renal osteodystrophy?
Increased serum phosphate | Reduction in calcitriol
56
Describe the sequelae of renal osteodystrophy.
1. Secondary hyperparathyroidism (compensatory) 2. This is unsuccessful + hypocalcaemia develops 3. This leads to excess stimulation of the parathyroid glands, leading to parathyroid hyperplasia 4. Parathyroid cells begin to reduce expression of calcium-sensing receptors (CSR) + Vitamin D receptors (VDR) + become autonomous (tertiary) 5. This causes hypercalcaemia
57
What symptoms are common in metabolic bone disease?
Hypo/Hypercalcaemia Hypo/Hyperphosphataemia Bone pain (fractures + deformity)
58
What can effect peak bone mass?
Growth | Exercise
59
What is the lifespan of osteoclasts and osteoblasts?
Osteoclasts ~ 2 weeks | Osteoblasts ~ 3 months
60
What are the 3 main systems involved in calcium balance?
GUT: Ingested Ca Kidney: Excreted Ca Bone: Flux (compensatory mechanism)
61
What is the set-point on a PTH/ Ca2+ graph?
Point of 1/2 max. suppression of PTH, steep part of slope. | Small shift in Ca2+ causes large change in PTH
62
How does PTH cause bone resorption?
Through acting on receptors on osteoblasts Osteoblasts release RANKL RANKL binds RANK on osteoclast precursors, causing proliferation + differentiation
63
Why can high serum calcium be a medical emergency?
It causes a diuresis High Ca sensed on basolateral membrane shuts down Na+/ K+ recycling Less Na+ reabsorbed, thus less water reabsorbed Increases dehydration, Ca keeps increasing
64
How do acute and chronically raised PTH differ in effect?
Acute: Anabolic Chronic: Catabolic, increases cortical bone resorption
65
What are the feedback actions of Calcitriol?
Negative feedback to parathyroid directly to reduce PTH secretion Positive feedback to bone to increase FGF23
66
What 5 consequences can arise from hypocalcaemia that may develop in osteomalacia?
``` Paresthesias Bronchospasm's Seizures Laryngospasms Heart failure ```
67
How is PTH stimulated phosphate absorption in the gut compensated for?
FGF23 causes phosphate excretion in PCT
68
How do skeletal remodelling disorders caused by CKD contribute to mortality rates?
CKD impairs skeletal anabolism, decreasing osteoblast function + bone formation rates CKD contributes to heterotopic calcification (vascular= strokes)

YR2: Musculoskeletal (10 decks)

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