methods Flashcards
(27 cards)
1
Q
why did you use yeast as a model system
A
2
Q
disadvantages of yeast as a model system
A
3
Q
Why SEY6210?
A
4
Q
imaging - why used?
A
5
Q
endogenous tagging - method?
A
6
Q
transformation - method?
A
7
Q
plasmids - method?
A
8
Q
plasmids - advantages?
A
9
Q
plasmids - disadvantages?
A
10
Q
BIFC - technique?
A
11
Q
BIFC - alternatives - why not?
A
12
Q
FRAP- technique?
A
13
Q
protein purification - technique?
A
14
Q
NBD-labelling - technique/science?
A
15
Q
PI4P transfer assay - technique?
A
16
Q
why stopped using PI4P transfer assays?
A
17
Q
PM integrity - technique/science?
A
18
Q
GCAMP better than dye
A
19
Q
HPLC - technique?
A
20
Q
Mass spectrometry - technique?
A
21
Q
lipotype vs nozumo?
A
22
Q
cryo-ET
A
23
Q
chapter 3 -summarise
A
24
Q
chapter 3 - one sentence
A
25
chapter 3 - key result
26
chapter 3 - novelty
27
chapter 3 -previous data that inspired this work?
