Methods for Inhibiting Microbial Growth Flashcards
the treatment of an object or surface to make it safe to
handle
Decontamination
a process that directly targets pathogens although it
may not eliminate all microorganisms
Disinfection
Disinfection requires agents called __________ that actually kill microorganisms or severely inhibit their growth.
disinfectants
heat, radiation, and filtration
Physical Method
chemical antimicrobial agents
Chemical Methods
➢a sealed heating device that
uses steam under pressure to
kill microorganisms
Autoclave
At ______°C, the time to achieve
sterilization of small amounts of
endospore-containing material
is about ____min.
121;15
uses heat to significantly reduce rather than totally eliminate the microorganisms found in liquids, such as milk.
Pasteurization
useful for disinfecting surfaces and air,
and is widely used to decontaminate and disinfect the
work surface of laboratory laminar flow hoods
UV radiation
electromagnetic radiation of sufficient energy to produce ions and other reactive molecular species from molecules with which the radiation particles collide
Ionizing Radiation
Heat-sensitive liquids not subject to ionizing radiation
are typically sterilized by
Filter Sterilization
Types of filters in Filter Sterilization:
1) Depth filters
2) Membrane filters
3) Nucleopore filters
a fibrous sheet made from an array of overlapping paper or glass fibers that traps particles in the network of fibers
Depth filters
➢the most common filters used for
liquid sterilization in the
microbiology laboratory
➢composed of high-tensile strength
polymers manufactured in such a
way as to contain a large number
of tiny pores.
Membrane filters
➢made from a thin
polycarbonate film that is
treated with radiation and then
etched with a chemical,
yielding very uniform holes
➢commonly used to isolate
specimens for scanning electron
microscopy
Nucleopore filters
