Methods In Haem 2 + Leukemias 2 & 3

Question 1

Describe technologies commonly used in hematological diagnosis and application to particular disease states(example).

Question 2

Compare Synchronised VS Un-Synchronised technique.

Question 3

Brief notes on staining.

Question 4

Explain the mechanism of FISH.

Question 5

What is the diagnosis/clinical managements of CML, AML, CLL, and MCL.

Question 6

Brief notes on PCR and Rq-PCR.

Question 7

Define flow cytometry and mechanisms behind it.

Question 8

What are the five come pats of flow cyt?

Question 9

Outline gating and immuniphenotyping …

Question 10

What are the advantages and disadvantages of each technique in diagnosis?

Question 11

Why is cytogenetics and MRD analysis by RQ-PCR used in tandem for follow up?

Question 12

Understand the pathogenesis and prognosis of Acute Myeloid Leukemia (AML). Explain AML.

Question 13

What are some prognostic indicates of AML? What its incidence in populations?

Question 14

Outline and compare AML classifications from WHO and FAB.

Question 15

Explain the core binding factor (CBF) and its relevant mechanism.

Question 16

Explain the CBF mechanism in Patients with AML stage M2: t(8;21) RUNX1

Question 17

Explain the CBF mechanism Patients with AML stage M4: t(6;16) or inversion

Question 18

Outline the therapies used in AML patients (except M3) + prognosis.

Question 19

Explain the relevance of retinoic acid.