METHODS OF BLOOD COLLECTION

Question 1

What method of blood collection?
(a) A method of blood collection by skin puncture. Method of choice in pediatric patients. It is useful in adults with extreme obesity, severe burns
and thrombotic tendencies.*
(b) Blood collected from the heel, finger, earlobe or toe.
(c) In older infants and elderly population, the palmer (fleshy) surface of the distal phalanx of the middle or ring finger, with the middle finger being the first choice.

Answer 1

Capillary Puncture

Question 2

What method of blood collection?

(a) A blood collection procedure via the patient’s vein.
(b) Best method of blood collection.
(c) The relative ease of obtaining venous blood makes this a primary source of specimen for clinical laboratory analyses.

Answer 2

Venipuncture Method

Question 3

Method of choice in pediatric patients

Answer 3

Capillary Puncture

Question 4

Can you draw blood from IV sites?

Answer 4

No

Question 5

Why cant you draw blood from IV sites?

Answer 5

It dilutes the specimen.

Alters test results.

Question 6

A liquid anticoagulant used in purple tops.

_______ is a chelating agent that binds calcium so blood cannot clot.

Answer 6

EDTA

Ethylenediaminetetracetic Acid

Question 7

CBC/ Differential WBC can be refrigerated for how long?

Answer 7

24 hours

Question 8

A powder anticoagulant used in light blue tops

Answer 8

Sodium Citrate

Question 9

Sodium Citrate

Typically used for what coagulation studies:

Answer 9

a) Prothrombin Time (PT).

b) Partial Thromboplastin Time (PTT).

Question 10

What anticoagulant
A liquid or powder anticoagulant in green tops.
Typically used for plasma studies and Chemistry testing

Answer 10

Heparin

Question 11

What color tube has no anticoagulant or no additives.
Used mainly for:
(1 Chemistry
(2 Serology
(3 Blood bank
(4 Other tests requiring serum.

Answer 11

Red top tube

Question 12

What tube?

a) No anticoagulant.
b) Contains a serum separator gel.
c) Primarily used for chemistry testing.

Answer 12

Serum separator tube (SST)/Gold Top, Tiger top, HIV tube (red/yellow).

Question 13

What tube top?

a) Contains sodium fluoride
b) Used for glucose studies.
c) ETOH level analysis.
d) Inhibits Glycolysis.

Answer 13

Grey top tube

Question 14

What is the draw order?

Answer 14

blood culture tubes,
blue top, 
red top, 
SST, 
green top, 
lavender top, 
gray top.

Question 15

What do you do for a pt with syncope from blood draw?

Answer 15

Have patient lie down.

Treat symptomatically

Question 16

True/False

You can draw blood while the patient is standing up

Answer 16

FALSE

Never draw blood while patient is standing up

Question 17

Green top tubes are used for what studies?

Answer 17

Chemistry
Ammonia
Adrenocorticotropic hormone (ACTH)

Question 18

Not mixing the tube results in what?

Answer 18

Blood clots

Question 19

What blood draw error

• Could render the specimen unacceptable due to quantity not sufficient to perform the test.
• Results in improper anticoagulant/blood ratio.

Answer 19

Short fill