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Microbiology and Parasitology > Methods of Studyinh Microorganisms > Flashcards

Methods of Studyinh Microorganisms Flashcards

1
Q

It allows small numbers of organisms present in a sample viable for laboratory evaluation

A

Culture or Cultivation

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2
Q

What are the general methods of study?

A 
Microscopic Examination
Culture or Cultivation
Biochemical tests
Animal Inoculation
Serologic Tests
Molecular Techniques
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3
Q

It is the use of commercially available kit

A

Enterotube System

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4
Q

Types of Stain used

A

Simple Stain
Differential Stain
Negative Stain
Special Stain

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5
Q

Another term for molecular techniques

A

Nucleic Acid-based methods

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6
Q

Preparation of a bacterial smear

A

Thin smear
Air dried
Heat-fixed

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7
Q

3 Categories for Molecular Technique

A

Hybridization
Amplification
Sequencing and Enzymatic Digestion

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8
Q

Period of incubation

A

24-48 hrs

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9
Q

Types of culture accdg to composition

A

Synthetic
Nonsynthetic
Living tissue

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10
Q

Special stains

A 
Dorner
Anthony, Hiss
Leiffson
Gray
Albert’s Methylene Blue
Fontana Trebondeau
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11
Q

Gram (+)

A

Blue or violet

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12
Q

Gram (-)

A

Red color

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13
Q

Rationale for acid-fast organism is red

A

Presence of mycoluc acid

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14
Q

Component of Differential Gram Stain

A

Hucker

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15
Q

Component of Differential Acid Fast stain

A

Zeihl Neelsen

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16
Q

Gram’s rule

A

All cocci are positive except Branhamella, Moraxella, Veilonella and Neisseria
All bacilli are negative except Clostridium, Genus Bacillus, Corynebacterium and Mycobacterium

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17
Q

Microscopic Examination is also known as

A

Visualization or Optical method

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18
Q

Tests that are used to identify organisms grown in culture

A

Biochemical tests

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19
Q

Type of immunity in which you are involved in producing antibody

A

Active

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20
Q

The visible mass of growth of a microorganism

A

colony

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21
Q

Stain used in Negative stain

A

India ink

10% Nigrosin dye

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22
Q

Types microscope used

A

Darkfield
Brightfield
Electron

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23
Q

The term being used which is associated with the conventional culture methods

A

Phenotype

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24
Q

It is a foreign object described to be non-self entering the host body to elicit antibody formation

A

Antigen

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25
Uses transmitted light
Brightfield
26
Uses reflected light
Darkfield
27
Dorner
Spores
28
Leiffson
Flagella
29
Fontana Trebondeau
Treponema
30
Rational on why is it gram negative
Presence of lipids
31
What decolorizer is used in the acid-fast chain?
Acid alcohol
32
What primary stain is used in the Gram stain?
Crystal violet
33
What mordant is used in the gram stain?
Gram’s iodine
34
Rationale why gram positive is blue or violet
Presence of protein Mg ribonucleate
35
Counterstain of acid fast chain
Methylene blue
36
Primary stain of Acid fast chain
Carbol Fuschin
37
Gray
Flagella
38
Anthony, Hiss
Capsule
39
Presence of colony
Solid medium
40
Types of culture medium accdg to physical state
Solid medium Semisolid medium Broth medium
41
Examples of broth medium
Urea and lactose broth
42
Other term for solid medium
Agar medium
43
How many percent of agar does semi solid has?
0.3-0.5%
44
How many percent of agar does solid medium has?
1-2%
45
Ideal temp for bacteria to grow
37 degrees celsius
46
Turbidity, change in color, presence of bubble or gas
Broth medium
47
Example of semi solid medium
Sulfide Indole Motility medium
48
Counter stain of gram stain method
Safranin
49
It is the substance produced by the immune system as a result of antigenic stimulation and is specific to the antigen
Antibody
50
Term being used which means to characterize etiologic agents of diseases
Genotype
51
Analysis of the microbial DNA or RNA
Molecular technique
52
Uses the concept of immunology
Serologic tests
53
An artificial substance which supports the growth of the organism
Culture medium
54
Involves in the isolation of the organism
Culture or Cultivation
55
Commonly used methods in the laboratory
Microscopic Culture Biochemical
56
Albert’s Methylene Blue
Cytoplasmic Granules
57
Uses stained or unstained smears
Microscopic examination
58
A grape smell bacteria
Pseudomonas aeruginosa
59
Used to identify the organisms grown in culture
Biochemical tests
60
Used to demonstrate virulence
Animal Inoculation
61
Best blood host
Sheep
62
New methods
Serologic test | Molecular techniques
63
Demonstrate effects of drugs
Animal inoculation
64
Animal inoculation is used in the making of ___ & ____
Antiserum ans antitoxins
65
Demonstrating the presence of antigen and antibody in the blood of patient
Serologic tests
66
Rationale why smears must be air dried
To maintain the bacterial morphology
67
Rationale why smears must be heat-fixed
Kills organism and allows smear to adhere on the slide
68
Rationale why smear must be thin
Avoid overlapping of cells | Avoid washing out smears
69
Phases of microscopic study
Use of stained organisms | Use of unstained organisms
70
Microscope used for the study of viruses and parasites
Electron
71
Microscope used for the study of spirochetes
Darkfield
72
Microscope used for the study of bacteria, fungi and paeasites
Brightfield
73
It offers a manner of studying the organism in its living state
Use of unstained organisms
74
Example of using the unstained organisms
Wet mount Hanging drop Darkfield Mx
75
Newest advances in laboratory diagnosis
Molecular techniques
76
Stain used on simple stain
Crystal violet | Safranin
77
Stain used in differential stain
Gram stain | Acid fast stain
78
Another term for broth medium
Liquid medium
79
Type of immunity where antibody is preformed
Passive
80
Enzyme systems
Coagulase
81
It offers a manner of grouping the organisms based on their morphology
Microscopic examination

Microbiology and Parasitology (2 decks)

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