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MIC 206 > MIC 206 Module 4 > Flashcards

MIC 206 Module 4 Flashcards

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1
Q

What is the purpose of the motility test?

A

The motility test is used to determine the motility of an organism.

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2
Q

What type of media is used in the motility test?

A

MTM media is a semi-solid agar with a colorless indicator dye.

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3
Q

How is the motility test performed?

A

Inoculate the media with the microorganism using a sterile inoculating needle and stab the media about two thirds of the way down.

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4
Q

What indicates bacterial growth in the motility test?

A

Bacterial growth is detected by the presence of red color produced by the reduction of the dye.

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5
Q

What should be observed after incubating the inoculated tube?

A

Observe the media for bacterial growth; non-motile bacteria will show red dye only in the stab line, while motile bacteria will diffuse the dye.

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6
Q

How do you record the results of the motility test?

A

Record the reaction for motility or non-motility based on the diffusion of the red dye.

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7
Q

What does a negative result look like in the motility test?

A

A negative result is indicated by a sharp line where bacteria do not move along the line of inoculation.

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8
Q

What does a positive result look like in the motility test?

A

A positive result is indicated by diffusion of bacteria from the sharp line of inoculation, which can be partial or complete.

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9
Q

What is the purpose of the lactose fermentation test?

A

To determine if bacteria can ferment lactose into acidic end products.

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10
Q

What does the lactose fermentation broth contain?

A

Lactose sugar and a pH indicator.

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11
Q

What color change indicates a positive result in the lactose fermentation test?

A

The broth changes from salmon-pink to yellow.

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12
Q

What is the first step in the protocol for the lactose fermentation test?

A

Inoculate a tube of lactose broth with bacteria using a sterile inoculating loop.

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13
Q

What should be done after inoculating the lactose broth?

A

Incubate the inoculated tube until the next lab session.

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14
Q

How do you observe the results of the lactose fermentation test?

A

Observe the reaction for color change.

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15
Q

What does a negative result look like in the lactose fermentation test?

A

The lactose broth does not change color and remains salmon-pink.

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16
Q

What does a positive result look like in the lactose fermentation test?

A

The broth changes to a yellow color.

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17
Q

What does the urease test detect?

A

The ability of bacteria to produce the enzyme urease when inoculated into the urea slant.

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18
Q

What does urease do in the urea slant?

A

Urease hydrolyzes urea to produce ammonia.

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19
Q

What is the result of ammonia production in the urease test?

A

The formation of ammonia changes the phenol red indicator to hot pink.

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20
Q

What is the first step of the urease test protocol?

A

Inoculate a tube of urea slant with bacteria using a sterile inoculating loop.

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21
Q

What should be done after inoculating the urea slant?

A

Incubate the inoculated tube until the next lab session.

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22
Q

How do you observe the results of the urease test?

A

Observe the reaction for color change.

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23
Q

What does a negative result look like in the urease test?

A

The urea slant does not change color and remains yellow.

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24
Q

What does a positive result look like in the urease test?

A

The slant changes to a hot pink color.

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25
What is the purpose of the Citrate test?
To determine if an organism uses citrate as its only carbon source.
26
What happens to bacteria that cannot use citrate in the Citrate agar?
They will not grow in the Citrate agar.
27
What indicates that bacteria can use citrate as a carbon source?
The agar changes color from green to blue.
28
What is the first step in the Citrate test protocol?
Inoculate a Citrate agar tube with bacteria using a sterile inoculating loop.
29
What should be done after inoculating the Citrate agar tube?
Incubate the inoculated tube in the class test tube rack until the next lab session.
30
How do you observe the results of the Citrate test?
Observe the reaction for color change.
31
What does a negative result look like in the Citrate test?
The Citrate agar does not change color.
32
What does a positive result look like in the Citrate test?
The agar changes to a blue color.
33
What is the purpose of the Methyl Red-Voges Prausker (MR-VP) Test?
The MRVP test is used to determine the end products of glucose fermentation.
34
What are the two types of end products that bacteria can produce from glucose fermentation?
Some bacteria ferment glucose to make mixed acids, while others make 2,3 butanediol.
35
How are mixed acids detected in the MRVP test?
Mixed acids are detected in the MR test.
36
How is 2,3 butanediol tested in the MRVP test?
2,3 butanediol is tested by the VP test.
37
What is the first step in the MRVP test protocol?
Inoculate an MRVP tube with bacteria using a sterile inoculating loop.
38
What is the incubation time and temperature for the MRVP tube?
Incubate the inoculated tube for 48 hours at 37 degrees.
39
What should be done after 48 hours of incubation?
Check for growth and split the MRVP culture into two aliquots, labeling one as MR and the other as VP.
40
What is added to the MR aliquot during the MR test?
Add 10 drops of MR reagent to the MR aliquot.
41
How long should the MR aliquot be incubated after adding the MR reagent?
Incubate for 10 minutes.
42
What should be done if the MR test results are negative?
Use the other aliquot to test for VP.
43
What reagents are added to the VP aliquot?
Add 12 drops of Voges-Prausker reagent A and 4 drops of Voges-Prausker reagent B.
44
How long should the VP aliquot be incubated after adding the reagents?
Incubate for up to one hour at room temperature.
45
What indicates a positive result in the MR test?
A positive result is seen when the broth changes to a light pink to red color.
46
What indicates a negative result in the MR test?
A negative result is seen when the MR broth does not change color.
47
What indicates a positive result in the VP test?
A positive result is seen when the broth changes to a brownish-red color.
48
What indicates a negative result in the VP test?
A negative result is seen when the VP broth does not change color.
49
What does SIM stand for in microbiology?
SIM stands for sulfide, indole, motility.
50
What type of medium is SIM?
SIM is a multi-test medium that tests more than one aspect of a bacterium’s metabolism.
51
What three aspects does SIM medium test?
SIM medium tests for the production of hydrogen sulfide, the formation of indole, and motility.
52
What is the first step in the SIM medium procedure?
Obtain a deep of SIM medium.
53
How should the inoculating needle be used in the SIM medium procedure?
Stab the medium about 2/3 of the way down and out the same pathway as quickly as possible with your assigned organism.
54
How long should the SIM medium be incubated?
Incubate the tube for at least 48 hours.
55
How is hydrogen sulfide production indicated in SIM medium?
Hydrogen sulfide production is indicated by a black color in the medium.
56
How is indole production indicated in SIM medium?
Indole production is indicated by a red ring on the surface of the deep after adding Kovac’s reagent.
57
How is motility indicated in SIM medium?
Motility is indicated by the ability of the organism to ‘fan’ away from the streak or if the entire tube appears cloudy.
58
What does non-motile growth look like in SIM medium?
If the organism is non-motile, the growth will only appear along the stab line.
59
What does a black color in SIM medium indicate?
A black color indicates hydrogen sulfide production.
60
What does a red ring in SIM medium indicate?
A red ring indicates indole production.
61
What does cloudy appearance in SIM medium indicate?
A cloudy appearance indicates motility.
62
What are the results for bacterial sample A in the biochemical tests?
Motility: Negative Sulfur reduction: Negative Indole production: Positive Lactose fermentation: Negative Citrate utilization: Negative MR test: Positive VP test: Negative Urease: Negative
63
What are the results for bacterial sample B in the biochemical tests?
Motility: Positive Sulfur reduction: Positive Indole production: Negative Lactose fermentation: Negative Citrate utilization: Positive MR test: Positive VP test: Negative Urease: Negative
64
What are the results for bacterial sample D in the biochemical tests?
Motility: Negative Sulfur reduction: Positive Indole production: Positive Lactose fermentation: Negative Citrate utilization: Negative MR test: Positive VP test: Negative Urease: Positive
65
What are the results for bacterial sample E in the biochemical tests?
Motility: Positive Sulfur reduction: Negative Indole production: Negative Lactose fermentation: Positive Citrate utilization: Positive MR test: Negative VP test: Positive Urease: Negative
66
What are the results for bacterial sample H in the biochemical tests?
Motility: Positive Sulfur reduction: Negative Indole production: Positive Lactose fermentation: Positive Citrate utilization: Negative MR test: Positive VP test: Negative Urease: Negative
67
What is the identity of bacterial sample A?
Shigella flexneri
68
What is the identity of bacterial sample B?
Salmonella typhimurium
69
What is the identity of bacterial sample D?
Shigella flexneri
70
What is the identity of bacterial sample E?
Enterobacter aerogenes
71
What is the identity of bacterial sample H?
Escherichia coli

MIC 206 (3 decks)

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