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Question 1

Feedback inhibition is a regulatory mechanism of gene expression that operates at which level?

Answer 1

Enzymatic level

Question 2

Transcription of the lac operon is subject to which regulatory mechanisms?

Answer 2

Gene induction

Question 3

Catabolite repression controls which genetic elements?

Answer 3

Various genes and operons

Question 4

Transformation is?

Answer 4

DNA uptake from the environment by a bacterium

Question 5

Generalised transduction is carried out by

Answer 5

Lytic bacteriophage

Question 6

The restriction enzyme EcoRI cleaves the sequence 5’-GAATTC-3’ to generate DNA fragments with

Answer 6

A 5’ single stranded overhang

Question 7

If a circular plasmid containing 3 EcoRI sites and 2 PvuII sites is restricted (or cut) using these two enzymes, the number of fragments generated is:

Answer 7

6

Question 8

If a restriction enzyme (of the type II) recognizes a 7 base pair sequence, this sequence is expected to occur once in every

Answer 8

16384 base pairs

Question 9

Cloning vectors are typically

Answer 9

Small, autonomously replicating, medium to high copy number plasmids that contain restriction sites for cloning

Question 10

The scientist and Nobel laureate Frederick Sanger developed

Answer 10

Dideoxy DNA sequencing

Question 11

Which of the following techniques has been used for gene mapping

Answer 11

Conjugation

Question 12

The lac operon is subject to two different types of transcriptional control, which are

Answer 12

Gene induction and catabolite repression

Question 13

During conjugation DNA moves into the recipient cell as

Answer 13

Single stranded DNA in a 5’ to 3’ direction

Question 14

A biolistic delivery system or gene gun is an instrument used for

Answer 14

Artificial transformation

Question 15

An attenuator or leader sequence is typically found in

Answer 15

Amino acid biosynthetic operons

Question 16

Which naturally occurring phenomenon allowed scientists to show that DNA is the coding molecule?

Answer 16

Transformation

Question 17

Western blotting is based on

Answer 17

Protein-antibody interactions

Question 18

SMRT and Nanopore are two particular techniques used for

Answer 18

DNA sequencing

Question 19

During agarose gel electrophoresis DNA fragments separate

Answer 19

Based on their molecular weight

Question 20

The RecA protein is involved in

Answer 20

Integration of single stranded DNA into the resident chromosome during transformation

Question 21

In a double-stranded nucleic acid, cytosine typically base-pairs with:

Answer 21

b) guanine

Question 22

A major component of RNA but not of DNA is:

Answer 22

e) uracil

Question 23

Which of the following statements about the human genome is correct?

Answer 23

b) It encodes more RNA than proteins

Question 24

E. coli, DNA polymerase III:

Answer 24

c) is the principal DNA polymerase in chromosomal DNA replication.

Question 25

RNA Polymerase:

Answer 25

b) can synthesize RNA strands without a primer.

Question 26

Processing of a primary mRNA transcript in a eukaryotic cell does not normally involve:

Answer 26

b) conversion of normal bases to modified bases, such as inosine and pseudouridine.

Question 27

Compared with DNA polymerase, reverse transcriptase:

Answer 27

d) makes more errors because it lacks the 3' → 5' proofreading exonuclease activity.

Question 28

Which one of the following is true about the genetic code?

Answer 28

c) Several different codons may encode the same amino acid.

Question 29

Which of the following is/are true for protein synthesis in eukaryotes?

Answer 29

b) All proteins are initially synthesized with methionine at their N-terminus.