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micro bio midterm Flashcards

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1
Q

Bacteria that require oxygen to grow?

A

strict anaerobe

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2
Q

bacteria that tolerate oxygen but do not require it to grow?

A

intolerant anaerobe

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3
Q

What is the growth pattern of obligate anaerobes in thioglycollate media?

A

Gathers at the bottom of the tube

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4
Q

What is the growth pattern for faculatative anaerobes in thioglycollate media?

A

settles mostly at top but some of the bacteria is in the middle of the tube

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5
Q

What is a dormant form of bacterium that allows it to survive in nutrient poor conditions?

A

endospore

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6
Q

When using the highest magnification objective lens, what can be done to reduce the limit of resolution?

A

immersion oil

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7
Q

What does aseptic transfer mean?

A

transfer of bacteria in a sterile way without contamination

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8
Q

What mistake can be made while transferring from broth to TSA plate, if nothing grows on the plate?

A

The Luke could have been too hot, or the broth was not mixed up and all of the bacteria could have been settled at the bottom

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9
Q

what are the 4 ways to describe colony morphology?

A

Form, elevation, margin, color, translucent

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10
Q

What is the purpose of streaking TSA plates?

A

allows isolated colonies to grow

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11
Q

Some bacteria grow on top of a broth forming a type of surface membrane called what?

A

pellical

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12
Q

what term is used to describe when broth is clumpy

A

flocculent

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13
Q

Some motile microorganisms tend to produce what type of growth pattern on agar slants?

A

spreading edge

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14
Q

what does uniform turbidity mean?

A

All of the tubulars are spread evenly throughout

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15
Q

What is the genus of bacteria that produces endospores?

A

clostridium bacillus

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16
Q

What is a stain that is water soluble that has a low affinity for vegetative cells but binds to endospores?

A

malachite green

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17
Q

Name the genus of bacteria that can differentiate by acid fast staining?

A

mycobacterium

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18
Q

In acid fast stain carbolfuchsin binds to —— present in the cell wall of bacteria and stains the bacteria the color——

A

mycolic acid and red

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19
Q

Bacteria that do not grow in the presence of oxygen; it lethal to them

A

obligate anaerobe

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20
Q

Bacteria that can grow with or without oxygen but grows best with oxygen

A

facultative anaerobe

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21
Q

term used to describe bacteria that have a rod shape is —- or a spherical shape is ——

A

bacilli and cocci

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22
Q

For a simple stain the surface of a bacteria carries a —- charge and the chromogens of the basic stain carry a —- charge

A

negative and positive

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23
Q

If you preformed a gram stain on E. coli what would be the shape, color, and is it gram negative or positive

Study These Flashcards
A

rod shaped, red in color, and gram negative

24
Q

is Clostridium sporogeneses is an obligative anaerobe. Would you expect to see growth or culture when incubated under aerobic conditions?

25
how does a negative stain such as nigrosine work?
Negative stains do not dye the surface of the cell, so we are able to see the bacteria from the contrast dark background
26
What is the structure in the bacteria cell wall that determines the differential result of a Gram staining?
peptidoglycan
27
If you were to do a gram stain on staphylococcus epidermis what would be the shape color and is it gram negative or positive?
cocci shaped, gram positive, purple
28
What is a facultative anaerobe?
A bacteria that can live with or without oxygen
29
When describing cell arrangement, a cluster of spherical shaped cells is called ------ while a chain of spherical shaped cells is called ---
Staphylococcus and streptococcus
30
What color is phenol red at low pH (acidic)
pink
31
organisms grown on HEA that reduce sulfur compounds to H2S gas turn what color?
black
32
On HEA acid/base changes are detected by the pH indicators ---- (detects decrease pH) and ---- (detects increases pH)
ammonium citrate and sodium thiosulfate
33
Mannitol is a
pH indicator
34
What ingredient inhibits Gram negative bacteria growth?
Phenylethyl alcohol
35
What is MSA is it selective or differntial?
mannitol salt agar; selective and differential.
36
What is PEA selective or differential?
Phenylethylalchohol Agar, is selective
37
Is MacConkey agar selective or differential?
both
38
What is EMB and is it selective or differential?
Eosin methylene Blue, selective and differntial
39
What is HEA and is it selective or differential?
Hektoen Enteric agar, selective and differential
40
Which of the following will not grow on a PEA plate; S. epidermis, S. aureus, E. coli, or E. puecalis?
E. coli
41
Which of the following will not grow on a MSA plate, S. aureus, E. faecalis, E. coli, or S. epidermis?
E. coli and E. faecalis
42
Which of the following will not grow on a MEA plate; E. coli, E. faecalis, S. epidermis, or S. aureus?
E. faecalis and S. aureus
43
Which of the following will not grow on a EMB plate S. enteritis, S. aureus, E. coli, E. faecalis, or E. aerogenes?
S. aureus and E. faecalis
44
Which will not grow on HEA plate; E. coli, E. faecalis, S. enteritidis, or S. pexheri?
E. faecalis and E. faecalis
45
What color does S. epidermis stain on a MSA plate?
pink
46
What color does S. aureus stain on a MSA plate?
yellow
47
What color does S. enteritidis stain on a MEA plate?
white and cloudy
48
What color does S. enteritis appear on a EMB plate?
yellow
49
What color does E. aerogenes appear on a EMB plate?
red
50
What color does E. coli appear on a EMB plate?
purple
51
What color does E. coli appear on a HEA plate?
oragne
52
What color does S. . pexheri appear on a HEA plate?
green
53
What is the purpose of MSA plate?
encourage growth of a certain organism and inhibit the growth of another?
54
What is the purpose of a PEA plate
cultivate gram positive organisms .
55
What is the purpose of MacConkey Plate?
Differentiate members of the Enterobacteriaceae
56
What is the purpose of EMB plate?
detection and isolation of the gram negative bacteria.
57
What is the purpose of HEA plate?
Indicator of lactose fermentation and hydrogen sulfide production, and inhibits growth of gram-positive bacteria

micro bio (3 decks)

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