micro chapter 6 Flashcards
(21 cards)
Evolution
Major metabolic pathways and cellular structures evolved from early prokaryotic cells
Cell structure and Function
Microscopic eukaryotes (e.g., fungi, protozoa, algae) share processes with bacteria but have fundamentally different cellular properties
Metabolic Pathways
Bacteria and Archaea exhibit unique metabolic diversity (e.g., nitrogen fixation, methane production, anoxygenic photosynthesis). Microorganisms’ survival depends on their metabolic characteristics
Microbial Systems
Microorganisms are ubiquitous, living in diverse ecosystems, and are essential for biogeochemical cycles and microbiota
Energy Requirements
All living things need energy from light or chemical reactions, stored as ATP
ATP is used for locomotion, reproduction, nutrient transport, and synthesis of macromolecules
ATP structure
ATP consists of an adenosine backbone with three phosphate groups (alpha, beta, gamma).
High-energy bonds between the phosphate groups release energy when broken
ATP hydrolysis
ATP hydrolyzes quickly, transferring energy to drive endergonic reactions. The reaction is reversible
ATP + H20 —- ADP + Pi + free energy
Fueling Reactions
Produce reducing power in the form of NADH. Biosynthetic pathways use NADPH. ATP and reducing power are often used interchangeably in biological contexts
Substrate-level phosphorylation
Organic substrates become phosphorylated without energy input, transferring energy to ADP to form ATP
Glycolysis process
Glycolysis splits sugar molecules, transferring energy to ATP. It occurs in two phases:
Phase 1: Consumes 2 ATP (energy in).
Phase 2: Releases energy, producing 2 ATP and 2 NADH (energy out)
Fermentation
NADH returns electrons to pyruvate, producing organic acids or alcohols
Respiration
Electrons from donor molecules reduce NAD to NADH, generating a proton motive force for ATP production
Transmembrane Ion Gradients
Generated during respiration and photosynthesis, these gradients produce more energy but require more time and resources
Redox Tower
The hierarchy of electron acceptors affects energy harnessed during respiration. Microbes can utilize various electron acceptors, enhancing metabolic diversity
Aerobes
Require oxygen for metabolism and can detoxify reactive oxygen species (ROS)
Anaerobes
Do not require oxygen and are generally unable to detoxify ROS
Oxygenic Photosynthesis
Involves water as an electron donor, producing oxygen
Anoxygenic Photosynthesis
Uses other electron donors and does not produce oxygen
Transhydrogenases
convert NADH into NADPH, and ensure there is sufficient NAD+ to receive electrons
Chemotrophs
electron moves from high energy level in a chemical molecule (electron donor) to lower energy level in another molecule (electron acceptor) – oxidative
Phototrophs
light energy (i.e., photons) excites photosynthetic pigments and moves an electron to a higher energy orbital,