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Microbiology > Micro Exam I > Flashcards

Micro Exam I Flashcards

(91 cards)

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1
Q

Group A Beta Strep

A 
Gram +
Beta Hemolytic
Catalase =
Bacitracin +
Camp =
PYR +
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2
Q

Group B Beta Strep

A 
Gram +
Beta Hemolytic
Catalase =
Bacitracin =
Camp  +
PYR =
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3
Q

Alpha Strep Viridians

A 
Gram +
Alpha Hemolytic
Catalase =
Bile Esculin =
NaCl broth =
Optochin =
PYR =
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4
Q

Strep pneumoiae

A 
Gram +
Alpha Hemolytic
Catalase =
Bile Esculin =
NaCl broth =
Optochin +
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5
Q

Strep Enterococcus

A 
Gram +
Gamma Hemolytic
Catalase =
Bile Esculin +
Nacl +
Optochin =
PYR +
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6
Q

Staph Aureus

A 
Gram +
Catalase +
Coagulase +
Mannitol +
DNase +
BactiStaph +
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7
Q

Staph epidermidis

A 
Gram +
Catalase +
Coagulase =
Mannitol =
DNase =
BactiStaph =
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8
Q

Branhamella (Moraxella) Catarrahlis

A

Gram =
Oxidase +
all sugar negative

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9
Q

Neisseria Gonorrhaea

A

Gram =
Oxidase +
Dextrose positive

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10
Q

Neisseria Lactamica

A

Gram =
oxidase +
sucrose =

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11
Q

Neisseria Meningitidis

A 
Gram =
oxidase +
dextrose +
lactose =
maltose +
sucrose =
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12
Q

Neisseria Sicca

A

gram =
oxidase +
lactose =

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13
Q

Ender of spontaneous generation controversy

A

pasteur

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14
Q

Pasteur developed technique of pasteurization using what product

A

wine

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15
Q

who was the first surgeon to control infection with an antiseptic

A

Lord Lister

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16
Q

who was the first person to demonstrate the role of bacteria in infectious diseases

A

koch

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17
Q

what group of infectious agents were discovered by examining bacterial-free filtrates

A

viruses

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18
Q

the concept of immunization was first demonstrated by

A

jenner

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19
Q

bacteremia

A

bacteria is present in blood

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20
Q

sepsis

A

bacteria is present in blood but also multiplying and producing toxins

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21
Q

virulence

A

ability of an organism to cause disease

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22
Q

carrier

A

host that harbors a pathogenic organism, no symptoms but can pass on disease

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23
Q

obligate pathogen

A

always causes disease

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24
Q

opportunistic pathogen

A

only causes disease when host is compromised

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25
antiseptic
used on living tissue to stop organisms from reproducing
26
disinfectant
inhibits growth and reproduction of microorganisms not necessarily killing them
27
cell wall
confers shape gram + organisms: more peptoglycane / less lipid gram = orgn : less peptroglycane/ more lipid
28
cytoplasmic membrane
regulates transport membranes
29
mesosomes
may involve separation of nucleus during cell division
30
ribosomes
protein synthesis
31
pili
aid in attachment o surfaces composed of proteins
32
inclusions vacuoles
used for storage of cellular products and food reserve
33
nucleiod
genome of cell composed of DNA
34
amphitichous
one flagella at both ends
35
atrichous
no flagella
36
monotrichous
one flagella at each end
37
lophotrichous
more than one at one end
38
peritrichous
all over the cell
39
polar
same as monotrichous
40
bacterial spore characteristic
resistant to drying resistant to heat resistant to chemical disinfectants and radiation can withstand harsh environment will germinate in environemtn favorable to the vegetative cell not considered reproductive in bacteria
41
prokaryotic
don't have limiting membranes and have rigid cell wall
42
eukaryotic
have limiting membranes
43
what is the most specific basis for the taxonomy and classification of bacteria
nucleic acid and hemology
44
exponential growth phase
where testing should occur ** | bacterial numbers increase dramatically
45
log phase
0-4 hours | cells enlarge but no division
46
stationary phase
cell growth = cell death
47
death phase
cell death > cell increase
48
obligate aerobes
use only molecular oxygen as the final hydrogen acceptor in respiration
49
organic compounds
final hydrogen acceptor in fermentation
50
facultative
although is has a preference it can grow either way
51
mesophiles
organism that grows best at body temperature
52
streak plate method
most convenient method for obtaining a pure culture from a mixed culture
53
mordant
salt of metal used to give dye an attachment point
54
decolorizing agent
most critical step in gram stain
55
which stains are used to demonstrate the presence of acid-fast bacteria
zeihl-neelsen kinyoun method fluorochrome method
56
optimum cultivation of bacteria characteristics
``` moisture nutrition requirements proper osmotic pressure ph incubation tampurature O2 availability proper prep ```
57
melting point of agar
97C
58
gelling point of agar
45 C
59
sheep blood agar description
enriched differential when related to hemolysis
60
several aspects of quality control program in micro
monitoring equipment media, anti-microbial susceptibility testing reagents
61
filtration
how to sterilze media that cannot withstand high tempuratures
62
modes of action of disinfectants
denaturation of cellular protein damage to DNA reaction with thiol groups of enzymes
63
isopropyl alcohol
whats used to prepare skin for venipuncture
64
freshly prepared 10% chlorox
best disinfectant for hospital use
65
characteristics for acceptable clinical specimen
``` anatomical and surgical pathology blood cultures earswab eyeswab sterile container proper id TIME RECEIVED ```
66
how soon should all specimens be cultured
as soon as possible
67
sterile body areas
``` CSF blood joints body cavities gall bladder stomach and duodenum renal tract middle ear ```
68
nonsterile body areas
``` skin auditory canal eye mouth throat sputum large bowel vagina ```
69
micrococcus
strict aerobe nonpathogenic rare in human body
70
stephylococcus
aerobic ferment glucose clinically significant
71
gaffyka
``` yellow in tetrads ferment glucose cannot reduce NO3 to NO2 on skin and in upper RT rare disease association ```
72
sarcina
packets of 8 bright yellow nonpathogenic
73
peptococcus
obligate aerobes URT and genitourinary tract cause infection
74
catalase test
test used to distinguish staph from strep
75
tube coagulase test
test that determines between staph aureus and staph epi
76
tube coagulase test tests for
free coagulase
77
hyaluronidase
"spreading factor" | hydrolyzes intracellular matrix allowing organism to spread easily
78
staph aureus entertoxin characteristics
``` affinity for GI tract food poisoning heat stable resistant to pepsin/trypsin not destroyed by boiling ```
79
bacteriophage typing
technique used to track down specific strain of staph aureus responsible for outbreak of infection in the nursery or OR
80
staphylococcus diseases
``` osteomyelitis septic arthritis bacteremia meningitis boils pneumonia SBE ```
81
staph saprophyicus
resistant to novobiocin and nonhemolytic | grows up to p disc
82
strep agalactiae
belongs to group b strep
83
group d strep
responsible for uti and wound infections
84
primary virulence factor
role of the capsule in infections due to strep pneumiae
85
most common cause of SBE
alpha strep viridans
86
which strain of streptococcus is associated with infections due to mycoplasma pneumoniae
strep MG
87
what is the reagent used for the oxidase test
oxidase reagenttetra methyl-p-phenylenediamine dihydrochloride
88
what antibiotic's are found in MTM
vancomysin - inhibits gram + cholistine - inhibits gram = rods niastatin - inhibits fungi
89
infections caused by Neisseria honorrhaea
gonorrhea, arthritis, sepsis, neonatal opthalmia
90
what specimen is used to detect carriers of Neisseria minigitidis
throat culture
91
what enzyme is produced by strains of Neisseria that are resistant to penicillin
b latamse

Microbiology (6 decks)

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