Micro LAB

Question 1

Taxo N

Answer 1

Oxidase

Question 2

Taxo P

Answer 2

optochin

Question 3

Taxo A

Answer 3

Bacitracin

Question 4

S. pneumoniae medium + for swelling

Answer 4

Neufield Quellung

Question 5

Etiologic agent of whooping cough

Answer 5

Bordatella pertussis

Question 6

Culture media with Neutral Red

Answer 6

MAC
SSA

Question 7

Culture media with Phenol Red

Answer 7

MSA
XLD
CTA
Urease
TSI/KIA

Question 8

Culture media with Bromthymol Blue

Answer 8

Citrate
HEA
OF
TCBS

Question 9

Acid (Orange-yellow) bacteria

Answer 9

V. cholerae

Question 10

Alkaline (blue-green) bacteria

Answer 10

V. parahemolytics

Question 11

Rapid Lactose Fermenters

Answer 11

Escherichia coli
Klebsiella - mucoid
Enterobacter

Question 12

Late Lactose Fermenters

Answer 12

Citrobacter
Serratia
Salmonella arizonae
Shigella sonnei
Hafria
Yersinia

Question 13

INDOLE (+)

Answer 13

MPEKPECP

Question 14

METHYL RED (-) VP (+)

Answer 14

KEESH

Question 15

CITRATE (+)

Answer 15

PCHEEKSS

Question 16

RAPID UREASE (+)

Answer 16

PPM

Question 17

H2S (+)

Answer 17

Black

SPACE

Question 18

Mcfarland standard turbidity

Answer 18

0.5

Question 19

Method used for Mueller Hinton agar

Answer 19

Kirby-Bauer method (disk diffusion)

Question 20

Gram (-) cocci

Answer 20

NVM

Question 21

color of beta hemolytic pattern

Answer 21

Yellow

Question 22

color of alpha hemolytic pattern

Answer 22

colorless/green

Question 23

gamma hemolytic pattern

Answer 23

no pattern seen in gamma

Question 24

bacteria with double hemolytic pattern

Answer 24

C. perfringens

Question 25

Culture media for Brucella

Answer 25

Castaneda agar

Question 26

For beta hemolytic, which test is used for susceptibility

Answer 26

Bacitracin

Question 27

Bacitracin (S)

Answer 27

S. pyogenes

Question 28

Bacitracin (R)

Answer 28

Perform CAMP/Hippurate test

Question 29

CAMP/Hippurate test (+) Beta-hemolytic

Answer 29

S. agalactiae

Question 30

CAMP/Hippurate test (-) beta hemolytic

Answer 30

Bile Esculin Hydrolysis test Group D (+)

Question 31

which test to perform after Group D (+)

Answer 31

perform 6.5% NaCl/PYR

Question 32

6.5% NaCl/PYR (+)

Answer 32

Enterococcus

Question 33

6.5% NaCl/PYR (-)

Answer 33

S. bovis

Question 34

Arrowhead in CAMP beta hemolytic

Answer 34

S. aureus

Question 35

Double arrowhead in reverse CAMP beta hemolytic

Answer 35

C. perfringens A. haemolyticum

Question 36

box head in reverse CAMP beta hemolytic

Answer 36

L. monocytogenes

Question 37

which test to perform on Alpha-hemolytic pattern bacteria

Answer 37

Optochin

Question 38

Optochin (S)

Answer 38

S. pneumoniae

Question 39

Susceptibility test for S. pneumoniae alpha hemolytic

Answer 39

MH

Question 40

Optochin (R)

Answer 40

Perform Bile Esculin hydrolysis (S. viridans)

Question 41

What causes the D zone?

Answer 41

erm gene

Question 42

Biological indicator of autoclave

Answer 42

B. stearothermophilus

Question 43

Biological indicator of Ionizing radiation

Answer 43

B. pumilis

Question 44

Biological indicator of Dry Heat

Answer 44

B. subtilis var. niger

Question 45

Biological indicator of Ethylene Glycol

Answer 45

B. subtilis var. globitii

Question 46

Test for Helicobacter pylori

Answer 46

Urea breath test

Question 47

Double stranded DNA viruses

Answer 47

Pa Ad Po He He

Question 48

RNA viruses

Answer 48

Reoviridae

Question 49

What detects southern blot

Answer 49

DNA

Question 50

What detects northern blot

Answer 50

RNA

Question 51

what detects western blot

Answer 51

proteins

Question 52

example of selective media

Answer 52

MAC (only gram. neg)

Question 53

which media should be autoclaved first?

Answer 53

MAC TSA

Question 54

which media should be dispensed in bottle first?

Answer 54

THIO BHI Lauryl broth

Question 55

used to differentiate Neisseria

Answer 55

Carb fermentation

Question 56

AFB how many fields

Answer 56

300

Question 57

bac. no. of E. coli in stool

Answer 57

0157H7 Enteropathogenic E. coli