Micro Plates

Question 1

Supportive Media

Answer 1

Supports growth of most nonfastidious bacteria

Question 2

Enrichment Media

Answer 2

contains growth factors such as blood, vitamins and yeast extract

Question 3

Selective Media

Answer 3

Contains additives to inhibit growth of certain bacteria

Question 4

What additives are in selective media?

Answer 4

dyes, bile salts, alcohols, antibiotics, acids

Question 5

Differential Media

Answer 5

formulates to produce distinct colonial appearances based on chemical reactions such as lactose, fermentation and H2S production.

Question 6

Sheep Blood Agar

Answer 6

Enriched differential media based on hemolysis. Used mostly on Strep annd Staph.

Question 7

Chocolate Agar

Answer 7

Enrichment Media used for isolation of Neisseria and Haemophilus influenzae

Question 8

CNA (Columbia Colistin-Nalidixic acid agar)

Answer 8

Selective media with acid to suppress Gram neg bacteria. Isolates gram pos cocci

Question 9

Phenylethyl alcohol agar (PEA)

Answer 9

Selective media with alcohol to inhibit Proteus. isolation of gram pos cocci and anaerobic gram neg rods

Question 10

Selective Strep Agaar (SSA)

Answer 10

For isolation of group A strep; contains antibioltics to suppress throat flora

Question 11

Eosin methylene blue (EMB)

Answer 11

selective and differential; inhibit Gram pos: isolate gram neg rods by fermentation

Question 12

MacConkey (MAC)

Answer 12

selective and differential; isolation fof gram neg rods with bile salts and crystal violet. Fermenters are pink.

Question 13

Sorbitol MacConkey (SMAC)

Answer 13

Isolation of E.coli 0157:H7; cannot ferment: colorless colonies

Question 14

Hektoen Enteric Agar (HE)

Answer 14

Isolation of Salmonella and Shigella in stool

Question 15

Reactions of HE agar

Answer 15

Bile salts, bromothymol blue and acid fuchsin inhibit GI flora. Non pathogens are orange/pink. non lactose fermenters blue/green. H2S black precipitate.

Question 16

Xylose lysine deoxycholate (XLD)

Answer 16

isolation of Salmonella and Shigella in stool

Question 17

E. Coli on XLD

Answer 17

Yellow

Question 18

Proteus on XLD

Answer 18

Yellow with black centers

Question 19

Shigella on XLD

Answer 19

Colorless of Red colonies

Question 20

Salmonella on XLD

Answer 20

Red colonies with black centers

Question 21

Salmonella-Shigella agar (SS)

Answer 21

Isolation of Salmonella and Shigella in stool

brilliant green and bile salts inhibit other enterics.

Question 22

Salmonella on SS

Answer 22

black center from H2S

Question 23

Shigella on SS

Answer 23

colorless colonies

Question 24

Gram Negative Broth (GN)

Answer 24

isolation of Salmonella and Shigella from stools and rectal swabs

Question 25

Deoxycholate citrate agar

Answer 25

isolation of salmonella and shigella

Question 26

Campy Broth

Answer 26

isolation of Campylobacter from stool in overnight inc 4C

Question 27

Campy BAP

Answer 27

Isolation of Campylobacter from stool | incubate in gas chamber CO2 at 42C

Question 28

Salmonella on HE

Answer 28

Green with black centers H2S positive

Question 29

Shigella on HE

Answer 29

Green colonies

Question 30

Sabouraud Dextrose Brain Heart Infusion Agar (SabHI)

Answer 30

Primary recovery of saprophytic and dimorphic fungi

Question 31

Sabouraud Agar (SAB)

Answer 31

selective medium that is formulated to allow growth of fungi(dermatophytes) and inhibit the growth of bacteria.

Question 32

Brain heart Infustion agar (BHI)

Answer 32

a rich medium for bacteria, yeast, and pathogenic fungi; primary recovery of fungi and Actinomycetales

Question 33

Thioglycollate (THIO)

Answer 33

Grows aerobic, anaerobic and microaerophilic organisms

Question 34

Bile esculin azide w/ vanco

Answer 34

selective media for isolation of vanco resistant enterococci

Question 35

Spectra MRSA

Answer 35

isolation of oxacillin resistant S. aureus

Question 36

BCSA

Answer 36

Burkholderia cepacia Selective Agar for isolation of Burkholderia cepacia in CF patients

Question 37

Lim broth (LIM)

Answer 37

enrichment media enhances growth of group B strep

Question 38

Thayer Martin (TM)

Answer 38

selective agar for growth of pathogenic neisseria

Question 39

Bacteroides bile esculin (BBE)

Answer 39

isolation of B. fragilis

Question 40

ANA

Answer 40

isolation of anaerobic organisms | supplemented with Vit k1, hemin and cystine

Question 41

LKV

Answer 41

selective agar for isolation of anaerobic gram negative species specifically Bacteroides.

Question 42

Buffered charcoal yeast extract (BCYE)

Answer 42

isolation of legionella

Question 43

Mycobiotic Agar

Answer 43

isolation of pathogenic fungi from sources with bacteria

Question 44

Inhibitory Mold Agar

Answer 44

isolation of pathogenic fungi from sources with bacteria

Question 44

Bile esculin agar

Answer 44

Identify enterococcus and group D strep | Esculin is hydrolysis with iron and turns black