micro test 3 Flashcards

(51 cards)

1
Q

disinfection

A

reduces/destroys microbial growth

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2
Q

examples of disinfection

A

bleach, phenol (Lysol); cleans surfaces

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3
Q

sanitization

A

reduces/destroys microbial growth to public safe levels

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4
Q

examples of sanitization

A

detergent, cleaners; dishwashing, cleaning restrooms

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5
Q

sterilization

A

completely eliminates microbial growth and every other kind of growth

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6
Q

examples of sterilization

A

autoclaving, radiation; surgery prep

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7
Q

physical methods of control

A

dry heat, moist heat
refrigeration/freezing
pressure
desiccation (salt/sugar curing)
radiation (UV)
sonication (sound waves breaking holes in the microbes)
filtration (filtering a substance that can’t be heated)

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8
Q

dry heat e.g.

A

oven

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9
Q

moist heat e.g.

A

autoclaving, boiling, pasteurization

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10
Q

autoclave

A

121 degrees C for 15-20 min at 15 psi (sterilize)

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11
Q

boiling

A

sterilize

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12
Q

pasteurization

A

sanitize

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13
Q

Thermal Death Time

A

amount of time required to kill organism

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14
Q

Decimal Reduction

A

amount of time required to kill 90% organisms

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15
Q

Thermal Death Point

A

Temp required to kill all microbes in 10 min

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16
Q

static

A

slows growth/metabolism (bacteriostatic)

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17
Q

cidal

A

kills (bactericidal)

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18
Q

chemical methods of control

A

disinfectants
antiseptics
antimicrobials

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19
Q

disinfectants

A

high concentration used on hard surface e.g. Lysol

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20
Q

antiseptics

A

weaker than disinfectants used on people e.g. hydrogen peroxide

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21
Q

antimicrobials

A

taken internally (pill, IV, shot)

22
Q

types of antimicrobials

A

antibiotics
antivirals
antifungals
antiprotozoals
anthelminthics

23
Q

phenolics

A

e.g. Staphene & Lysol; work through membrane disruption & denature proteins

24
Q

heavy metals

A

e.g. copper, silver, nickel, zinc; antimicrobials work through charge disruption

25
alcohol
denatures membranes & proteins
26
"quats"
surfactants which use the disruption of membranes and water to cause cell lysis
27
bisbiguanide
membrane disruption
28
alkylating agents
formaldehyde; targets DNA & RNA
29
H2O2
hydrogen peroxide
30
CO2
carbon dioxide gas; makes carbonic acid & decrease cell pH
31
food preservatives
change pH; block enzymes function & cell wall synthesis
32
antibiotics
work against bacteria
33
Triazoles
target cell wall synthesis
34
Polyenes
cell membrane disruptors
35
Echinacnodins
target non-ergosterol cell wall components
36
Griseofulvins
cell divisions targeted
37
antiprotozoans
work against protozoans
38
naphaquinozone
mt disruptors (ATP synthesis)
39
sulfonamide
folic acid synthesis inhibitors
40
artemisinin
DNA synthesis
41
avermectins & benzimidazoles
targets nerves
42
antivirals
target viruses
43
acyclovir & AZT
nucleic acid bases (substitute for normal DNA & RNA)
44
etravirine
nucleic acid (interfere w/ replication)
45
amantadine & rimantadine
escape from phagocytosis
46
tamiflu & zanamavir
blocks neuramidase
47
pleconaril
viral uncoating
48
rltanvir & simepravir
protase
49
raltegravir
treat HIV; integrases
50
enfuvirtide
treat HIV; membrane fusion
51
antimicrobial testing
Kirby Bauer & E test strip