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Practical - Y1B3 (CVS & IBL) ๐Ÿ“˜ > Microbiology ๐Ÿฆ  > Flashcards

Microbiology ๐Ÿฆ  Flashcards

1
Q

What is the shape and stain of staphylococci?

A

Gram-positive cocci

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2
Q

What is the arrangement of staphylococci?

A

In Clusters

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3
Q

Are staphylococci motile?

A

No

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4
Q

Are staphylococci spore-forming?

A

No

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5
Q

What is the capsule of staphylococci?

A
  • S. aureus forms microcapsule

- Other species may produce slime layer

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6
Q

What are staphylococci classified into?

A

Coagulase positive: S. aureus.

Coagulase-negative: S. epidermidis and S. saprophyticus.

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7
Q

What are the samples used for the diagnosis of staphylococcus aureus?

A

Differ according to the site of infection (swabs, pus, sputum, C.S.F, blood, โ€ฆ).

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8
Q

What are the procedures of diagnosis of Staph aureus?

A

1- Sample
2- Direct film
3- Culture
4- Identification of colonies

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9
Q

Direct film of Staph aureus

A

Stained with Gram stain for characteristic morphology.

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10
Q

Culture of staph aureus

A
  • Facultative anaerobes, optimum temperature: 37 ยฐC, normal atmospheric CO2
  • Can grow on ordinary media.
  • S. aureus produce golden yellow endopigments.
  • S. aureus causes ฮฒ-hemolysis on blood agar.
  • Selective media is mannitol salt agar, where S.aureus ferments mannitol and salt inhibits other normal flora
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11
Q

How are colonies of staph aureus identified?

A
  • Film stained by Gram for characteristic morphology.
  • Biochemical reactions:
    โœ“ All staphylococci are catalase positive.
    โœ“ S. aureus is coagulase and DNAase positive and ferment mannitol.
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12
Q

What is catalase test used for?

A

.

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13
Q

What is the principle of catalase test?

A

.

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14
Q

What is the method of catalase test?

A

.

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15
Q

What is the interpretation of catalase test?

A

.

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16
Q

what is coagulase test used for?

A

.

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17
Q

What is the principle of coagulase test?

A

.

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18
Q

What are the types of coagulase?

A

.

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19
Q

What is DNAse agar used for?

A

.

20
Q

What is the principle of DNAse agar?

A

.

21
Q

What is the method of DNAse agar?

A

.

22
Q

What is the interpretation of DNAse agar?

A

.

23
Q

What is the significance of antimicrobial susceptibility?

A

.

24
Q

What is the significance of phage typing?

A

.

25
Q

What is a morphology of streptococci?

A

.

26
Q

What are the culture characters of streptococci?

A

.

27
Q

What is the difference between S. Pneumoniae and S. Viridans

A

.

28
Q

What is the classification of streptococci?

A

.

29
Q

What are the culture characters of streptococci?

A

.

30
Q

What is the media of streptococci?

A

.

31
Q

How is growth of streptococci identified?

A

.

32
Q

Serology of streptococci

A

.

33
Q

What is the method of bacitracin sensitivity test?

A

.

34
Q

What is the interpretation of bacitracin test

A

.

35
Q

How is the scarlet fever diagnosed?

A

.

36
Q

What is the principal and the method of Schultz-Charlton test?

A

.

37
Q

What are the uses of dick test?

A

.

38
Q

What is the principle of dick test?

A

.

39
Q

How is rheumatic fever diagnosed?

A

.

40
Q

What does a titer of 200 or more of ASO Todd units indicate?

A

.

41
Q

How is ASO titer measured?

A

.

42
Q

What are specimens used for diagnosis of Streptococcus pneumoniae?

A

.

43
Q

What are the characteristic morphology of streptococcus pneumoniae?

A

.

44
Q

What are the culture characters of Streptococcus pneumonia?

A

.

45
Q

How is growth of streptococcus pneumoniae identified?

A

.

46
Q

Pathogenicity to mice test

A

.

47
Q

Quellung reaction

A

.

Practical - Y1B3 (CVS & IBL) ๐Ÿ“˜ (8 decks)

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