Microbiology- bacteria Flashcards

(34 cards)

1
Q

What is the meaning of the word opportunistic pathogen?

A

only cause disease in compromised host, sometimes part of Normal Flora

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2
Q

What is the meaning of the word , virulence? How does LD 50 link in?

A

the quantitative ability of a microbe to cause disease

LD 50 stands for lethal dose 50 and indicates the dose of the organism required to kill 50% of the host under experimental conditions

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3
Q

What are Archaea bacteria?

A

live in extreme environments

not associated with disease in humans

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4
Q

What is the Type of DNA is found in bacteria?

A

Either a single circular chromosome or a plasmids (smaller circular DNA)

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5
Q

How do bacteria divide?

A

Binary fission

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6
Q

List 6 key features of bacteria? How do they help it be pathological

A
  1. No mitochondria (euk has)
  2. 70s ribosomes (euk,80s)
  3. Flagella- motility
  4. pili/fimbriae- adherence
  5. capsules- evade immune responce
  6. spores (resistant to physical and chemical agents)
  7. cell wall- protects against osmotic and mechanical shock. Adheres to host surfaces. protects against host immune system
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7
Q

What are the two main types of bacterial cell walls?

A

Gram positive

Gram negative

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8
Q

What is the difference between gram positive and gram negative bacteria?

A

Gram positive

  • Thick peptidoglycan
  • Techoic acid
  • No outer membrane

Gram negative

  • Thin peptidoglycan
  • porins
  • protein/lipids
  • outer membrane
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9
Q

What are the different shapes of bacteria? And which type of bacteria (positive or negative) do these shapes coincide with?

A

Cocci- Gram positive
Rod- positive and negtaive
Curved- Gram negative

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10
Q

What are the different shapes of bacteria? And which type of bacteria (positive or negative) do these shapes coincide with?

A

Cocci- Gram positive
Rod- positive and negative
Curved- Gram negative

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11
Q

What colour are gram negative and gram positive bacteria after staining?

A

Gram negative- Pink

Gram positive- purple- thick cell wall holds stain

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12
Q

How are gram positive cocci divided then further subdivided?

A

Catalase test-
Staphylococci are catalase positive
Streptococci are catalase negative

Staphylococci divided into- coagulase positive or negative

Streptococci divided into- beta-haemolytic, alpha-haemolytic and non-haemolytic

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13
Q

How are gram positive cocci divided then further subdivided?

A

Catalase test-
Staphylococci are catalase positive
Streptococci are catalase negative

Staphylococci divided into- coagulase positive or negative

Streptococci divided into- beta-haemolytic-
alpha-haemolytic and non-haemolytic
When grown in blood agar beta heamolytic will leave a zone of red cell lysis around each colony

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14
Q

Which bacteria causes scarlet fever? What are hall mark symptoms?

A

Streptococcus pyogenes
Strawberry tongue (rash)
Fever

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15
Q

Which bacteria causes Erysipelas?What are hall mark symptoms?

A

Streptococcus pyogenes
infection of upper dermis; skin rash on legs, toes, face, arms and fingers.
Fever, chills, fatigue, headaches

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16
Q

Which bacteria causes Cellulitis?What are hall mark symptoms?

A

Streptococcus pyogenes/Staphylococcus aureus
spreading infection of the soft tissues
red, hot, swollen and painful- extreme sepsis

17
Q

Which bacteria causes Carbuncle?What are hall mark symptoms?

A

Staphylococcus aureus
Cluster of boils
fever, general illness

18
Q

Which bacteria causes impetigo?What are hall mark symptoms?

A

caused by Staphylococci and or Streptococci

Skin infection

19
Q

Name 2 conditions caused by Staphylococcal enterotoxins

A

Acute staphylococcal enterocolitis

Staphylococcal “scalded skin” syndrome- upper skin layer lesions and high fever

20
Q

What causes Toxic Shock Syndrome? List some symptoms

A

Staphylococcal Superantigens

High fever, low Bp, coma

21
Q

Which bacteria causes chancre?What are hall mark symptoms?

A

Treponema pallidum

superficial skin lesion formed during syphilis

22
Q

How is Protozoa classified?

A
Motility 
pseudopodia
flagella
cilia
sporazoa (gliding)
23
Q

What is Plasmodium species (malaria) classified as?

A

Apicomplexa (SPOROZOA)

Protozoa disease

24
Q
What is Giardia (diarrhea, malabsorption)
 and Leishmania (leishmaniasis)
  classified as?
A

FLAGELLATES

Protozoa disease

25
What are the key features of fungi?
1. can be unicellular (yeasts) or multicellular (molds and mushrooms) 2. characteristics of plant life, but they are unable to perform photosynthesis 4. Cell wall- chitin 3. 80S ribose membrane bound organelles multiple linear, chromosomes some haploid, some diploid genes contain introns & exons DNA associated with histones
26
How does mold grow and reproduce?
grow as filaments (hyphae) which form a mesh (mycelium) Reproduce by forming spores which can cause infections
27
How does yeast divide?
divide by budding
28
Describe the main characteristic features of Helminths – parasitic worms
multicellular eukaryotic organisms lacking backbones, notochords, or exoskeletons. intestinal parasites They are divided into TREMATODES (flat non-segmented worms,flukes) Schistosomiasis disease CESTODES (flat segmented worms, tapeworms) NEMATODES (roundworms & filaria).
29
What is the meaning of the term normal microbiota and where can you find it?
Meaning- microbiota that establishes perminant colonies in or on the body without causing disease Locations- nose, somch, mouth, lungs, skin, gut vagina ureter
30
What is commensal bacteria?
Living in a relationship in which one organism derives food or other benefits from another organism without hurting or helping it.
31
What is the difference between Eukaryotes | and Prokaryotes? Which organisms fit into each category?
Prokaryotes- Contain DNA and RNA but no nuclear membrane and unicellular - Bacteria and archaea Eukaryotes- Possess DNA and RNA and DNA is enclosed in a nuclear membrane - Human cells. fungi, algae, helmonths, arthropods
32
How can Beta-haemolytic streptococci be further divided?
Groups A-G exist | depending on lance field antigen
33
How is Staphylococcus epidermis and aureus differentiated
Using mannitol salt agar Epidermis keeps agar pink Aureus turns it yellow
34
What causes Gas gangrene ? List some symptoms
- Clostridium perfringens | - Septic shock, organ failure