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Parasitology (LAB) > Microscope > Flashcards

Microscope Flashcards

(55 cards)

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1
Q

specimen is positioned properly on the stage of a microscope & illuminated by a light source will be magnified by a two-lens system

A

COMPOUND

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2
Q

magnified objects appear as dark objects against a bright background

A

BRIGHTFIELD

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3
Q

must exist between the magnified object & brightfield background for the objects to be visible

A

SUFFICIENT CONTRAST

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4
Q

Components of a Microcope:

A
  1. Lens System
  2. Illumination System
  3. Body
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5
Q

Further magnify the object for viewing

A

EYEPIECES OR OCULARS with 10x lenses

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6
Q

lenses magnify the intermediate image formed by the objective lenses

A

EYEPIECES OR OCULARS with 10x lenses

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7
Q

limit the area of visibility

A

EYEPIECES OR OCULARS with 10x lenses

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8
Q

should not be interchanged with other models of microscopes

A

EYEPIECES OR OCULARS with 10x lenses

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9
Q

used to adjust the lateral separation of the eyepieces for each individual

A

INTERPUPILLARY CONTROL

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10
Q

connects the eyepieces with the objective

A

OPTICAL TUBE

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11
Q

What is the standard length for the optical tube?

A

160 mm

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12
Q

gives structural site of attachment for the revolving nosepiece

A

NECK, OR ARM

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13
Q

the main vertical support of the microscope

A

STAND

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14
Q

supports: stage assembly, together w/ the condenser & base

A

STAND

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15
Q

holds the objectives

A

REVOLVING NOSEPIECE

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16
Q

allows easy rotation from one objective lens to another w/ a specific power of magnification

A

REVOLVING NOSEPIECE

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17
Q

Objective lens barrel are engraved w/ the power of magnification

A

`FOUR OBJECTIVE LENSES

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18
Q

Power magnification of the four objective lenses

A
  1. Power of magnification
  2. Numerical aperture (NA)
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19
Q

the larger ____, the greater the resolution, or ability to distinguish between fine details of two closely situated objects

A

NA

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20
Q

the larger _________, the smaller the viewing field

A

magnification

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21
Q

Standard powers of magnification:

A

Low power: 10X
High power: 40X
Oil immersion: 100X
lpo & hp: urine sediment examination

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22
Q

total magnification is calculated

A

10x (eyepiece) by 100x (oil immersion) = 1000x total magnification

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23
Q

shorter the wavelength of light, the greater the resolving power of the microscope

24
Q

supports the prepared microscope slide to be reviewed

25
engages coarse control (one direction)
FOCUS CONTROLS
26
engages fine control (moved in opposite direction)
FOCUS CONTROLS
27
Order of usage for focus controls:
Engage Coarse adjustment 1st then fine-tune w/ fine adjustment
28
gathers, organizes, & directs the light
CONDENSER
29
focuses light on specimen
CONDENSER
30
uniform illumination
CONDENSER
31
controls the angle (NA) & amount of light
IRIS
32
For best resolution, Iris is used:
Fully open Partially open Fully closed
33
For best resolution, Iris is used: sacrificed of image contrast
Fully open
34
For best resolution, Iris is used: slight improvement in image contrast
Partially open
35
For best resolution, Iris is used: Loss of resolution
Fully closed
36
is moved along an x- or y-axis
STAGE CONTROLS
37
when open: allows maximally sized circle of light
STAGE CONTROLS
38
when partially open: assists in centering the condenser apparatus
STAGE CONTROLS
39
2 types of Brightfield Illumination:
1. Critical illumination 2. Koehler (Köhler)System
40
results in increased but uneven brightness
Critical Illumination
41
light source focused on specimen
Critical Illumination
42
light source & condenser are properly aligned
Koehler (Köhler)System
43
evenly distributed brightness across the specimen
Koehler (Köhler)System
44
turns on light
RHEOSTAT/LIGHT CONTROL KNOB/LEVER
45
regulate brightness of light needed in visualizing specimen
RHEOSTAT/LIGHT CONTROL KNOB/LEVER
46
Care of the Microscope:
1. Carry microscope with 2 hands, support base with one hand 2. Always hold microscope in a vertical position 3. Only clean optical surfaces w/ a good quality lens tissue & commercial lens cleaner 4. Do not use the 10X & 40X objectives with oil 5. Clean the oil immersion lens after use 6. Always remove slides w/ the low-power objective raised 7. Store the microscope w/ the low-power objective in position & the stage centered
47
used to increase resolving power of light microscopes
IMMERSION OIL
48
increase the magnification & contrast of a specimen under a microscope
IMMERSION OIL
49
translates differences in phase of the light transmitted through or reflected by the object into differences of intensity of the image
PHASE CONTRAST MICROSCOPE
50
used for observing specimens that have not been stained & are in their natural state
PHASE CONTRAST MICROSCOPE
51
capable of providing information on absorption color & optical path boundaries between minerals of differing refractive indices
POLARIZED LIGHT MICROSCOPE
52
can distinguish between isotropic & anisotropic substances
POLARIZED LIGHT MICROSCOPE
53
takes advantage of oblique illumination to enhance contrast in specimens that are not imaged well under normal conditions
DARKFIELD MICROSCOPE
54
designed for directly studying the surfaces of solid objects
SCANNING ELECTRON MICROSCOPE (SEM)
55
used to view thin specimens (tissue sections, molecules, etc) through which electrons can pass generating a projection image
TRANSMISSION ELECTRON MICROSCOPE (TEM)

Parasitology (LAB) (3 decks)

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