MICROSCOPE

Question 1

Houses the optical parts found in the upper
part of the microscope

Answer 1

HEAD

Question 2

o Supports the microscope
o Houses the illuminator
o Must rest on a flat surface

Answer 2

BASE

Question 3

o Connects the head and the base
o When carrying the microscope, we hold the
arm with one hand, and the other hand
supporting the base

Answer 3

ARM

Question 4

o Another name for revolving nosepiece
o Holds the objective lens
o Used when switching objectives

Answer 4

Turrets

Question 5

o Characterized as a square platform with a
hole/opening at the center

Answer 5

STAGE

Question 6

opening at the center
o Where the slide with the specimen is
placed

Answer 6

APERTURE

Question 7

holds the slides in place

Answer 7

STAGE CLIPS

Question 8

allows you to move the stage
front or back

Answer 8

Y AXIS KNOBS

Question 9

allows you to move the stage
left or right

Answer 9

X AXIS KNOBS

Question 10

Used for the initial focusing of the
specimen

Answer 10

Coarse Adjustment knobs

Question 11

Objective lens: Scanner/LPO

Answer 11

Coarse Adjustment knobs

Question 12

Make the image more vivid

Answer 12

Fine adjustment knobs

Question 13

Objective lens: HPO/OIO
§ Do not use when using
Scanner/LPO

Answer 13

Fine adjustment knobs

Question 14

Holds the eyepieces in place

Answer 14

Eyepiece tube

Question 15

o Also known as eyepiece
o Remagnifies the imagine formed by your
objective lens
o 10x

Answer 15

Ocular lens

Question 16

o Allows us to compensate the difference in
eyesight between the left and right eye

Answer 16

Diopter Adjustment ring

Question 17

o Scanner - 4x;
o LPO - 10x;
o HPO - 40x;
o OIO - 100x;

Answer 17

red
yellow
blue
white

Objective lens

Question 18

is commonly used in
parasitology

Answer 18

LPO and HPO

Question 19

is used in parasitology when
determining the presence of parasites in
the blood (e.g. malarial parasites)

Answer 19

OIO

Question 20

o Used to collect and focus the light from the
light source/illuminator

Answer 20

Condenser

Question 21

o Located above the condense and below the
stage
o To control the amount of light that will reach
the specimen

Answer 21

Iris diaphragm

Question 22

Source of light that is located at the base of
the microscope

Answer 22

Illuminator/ Light Source

Question 23

Allows us to regulate the intensity of light in
the illuminator

Answer 23

Light intensity adjustment knob

Question 24

Controls the flow of current that allows the
light source to be turned on or off

Answer 24

Light siwtch

Question 25

Distance between the left and the right eye

Answer 25

Interpupillary distance

Question 26

When we change from one objective lens to another, the image will either stay in focus or close enough to being unfocused

Answer 26

Parfocal

Question 27

When we change from one objective lens to another, the image of the object will stay at the center

Answer 27

Parcentered

Question 28

This is the ability of the objective lens to view adjacent structures as separate objects

Answer 28

Resolving power

Question 29

- Distance between the objective lens and the closest surface of the cover slip - Increasing the objective lens will decrease the working distance

Answer 29

Working distance

Question 30

- Multiply the magnification power of the eyepiece to the objective lens that you are using - Eg. Using LPO: 10 x 10 = 100

Answer 30

Total magnification power

Question 31

It is called brightfield compound microscope because the magnified objects under the microscope appear dark against a bright background

Answer 31

BRIGHTFIELD COMPOUND MICROSCOPE

Question 32

because the image that we see is being magnified by two lenses (magnified twice: ocular lens and objective lens)

Answer 32

COMPOUND

Question 33

Give me the 5 microscope dos and dont's

Answer 33

1. Keep the microscope covered with a clean plastic or cloth cover when not in use. 2. Use a soft cloth dampened with xylene to clean immersion oil from the OIO. Then, polish with a clean lint free cloth. 3. When preparing the microscope for storage, make sure that the objective lens facing the stage is scanner and the stage is brought down 4. Manipulate the light intensity adjustment knob to reduce the light emitted by the illuminator to minimum; then, turn off the light switch and unplug.

Question 34

Give me the SLIDES DO’S AND DON’TS

Answer 34

1. Reject slides that are: ▪ imperfectly cleaned ▪ surface scratch or notched edge ▪ frosted appearance 2. Always handle cleaned slides by the edges. 3. Used slides should initially be placed in water containing a detergent. Then, slides will be cleaned one by one and transferred to a fresh solution of detergent. Rinse then dry with a clean cotton cloth 4. Slides are best stored in a dry place. 5. It is recommended that cleaned slides be stored by 10s

Question 35

Also known as pseudoparasites - Structures that resemble parasite but in reality are not (4)

Answer 35

ARTIFACTS AND CONFUSERS Disease process Medications Dietary habits Specimen contamination

Question 36

It is a technique used to measure the size of microscopic objects

Answer 36

Micrometry

Question 37

is an essential tool for characterization, classification, and identification

Answer 37

The size of a parasite:

Question 38

Give me the principle of micrometer

Answer 38

Calibration of the ocular micrometer using the stage micrometer

Question 39

A glass disc with 100 equal divisions or lines on it but with no absolute value and it is placed in the ocular of the microscope

Answer 39

Ocular micrometer

Question 40

are flat glass disk on which a line scale divided into 100 small division has been etched

Answer 40

ocular micrometer

Question 41

Distance between is _______, hence calibration is a must with the aid of an instrument known as ___________

Answer 41

unknown - stage micrometer

Question 42

- Used to calibrate ocular micrometer - It looks like a microscope slide but has a standard scale etched into it - The smallest division are 0.1mm in length (0.01 mm = 10um)

Answer 42

Stage micrometer

Question 43

The lens that is within the eyepiece of the light microscope is called the: scanning low power high power ocular

Answer 43

ocular

Question 44

2. The wheel under the stage that adjusts the amount of light is called the: coarse knob body tube stage clip diaphragm

Answer 44

diaphragm

Question 45

3. To focus a specimen, it is best to start with which objective: high power low power scanning ocular

Answer 45

scanning

Question 46

4. When using the high power objective, you should not adjust the: coarse focus fine focus diaphragm stage clips

Answer 46

coarse focus

Question 47

9. The scanning, low, and high power objectives are mounted on the: revolving nosepiece stage body tube eyepiece

Answer 47

revolving nosepiece

Question 48

10. A microscope has a 4x ocular lens and a 10x objective, what is this microscope's total magnification? 4x 14x 40x 400x

Answer 48

40X