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Botany Lecture > Microscope > Flashcards

Microscope Flashcards

(57 cards)

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1
Q

When was the first compound microscope was found

A

1590

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2
Q

He used a compound microscope to observe pores in cork; He called them “cells”

A

Robert Hooke

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3
Q

When did Robert Hooke used a compound microscope in observing pores in a cork?

A

1655

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4
Q

He is the 1st to see single-celled organisms in pond water.

A

Antoine Van Leeuwenhoek

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5
Q

Microscope History (MH) 1625

A

First use of term “microscope”

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6
Q

MH 1850

A

Opthalmoscope

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7
Q

MH 1873

A

Abbe equation EM

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8
Q

MH 1956

A

Optical Microscope, used by physician during eye surgery

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9
Q

MH 1957

A

Confocal principle

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10
Q

MH 1978

A

Confocal laser scanning microscopes

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11
Q

MH 1986

A

Nobel Prize Microscopy

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12
Q

MH 1990

A

Multiphoton microscopy

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13
Q

MH 1991

A

OCT

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14
Q

MH 1993

A

Super-resolution microscopy

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15
Q

MH 1994

A

STED/SIM

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16
Q

MH 2002

A

SD-OCT

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17
Q

MH 2006

A

RESOLFT, PALM, FPALM, STORM

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18
Q

MH 2008

A

Chemistry Nobel Prize GFP

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19
Q

MH 2014

A

Chemistry Nobel Prize super microscopes (OCTA)

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20
Q

It is a type of microscope that consists of a single lens set in a frame; it has low magnifiying power and enlarges an object without inverting the image

A

Simple or Magnifier

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21
Q

A type of microscope with two lenses system; the objective and eyepiece. It can magnify 100x.

A

Compound microscope

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22
Q

A type of microscope that is a simple microscope consisting of two microscopes mounted on a single body. See a real stereoscopic perception of depth.

A

Stereoscopic Microscope

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23
Q

A type of microscope which is a sophisticated apparatus which uses beams of electrons. Can magnify 100,000 times.

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A

Electron Microscope

24
Q

A type of microscope which is used for the study of detailed structure of surfaces of the specimen since image is formed by secondary electrons that are reflected back from the specimen. 3D.

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A

Scanning Electron Microscope (SEM)

25
A type of microscope which is exclusively used in examination of the internal structure of the cell since electrons that pass through the form the image.
Transmission Electron Microscope
26
The ratio of the apparent size of an object as seen through the microscope and actual size of the object.
Magnification
27
The adjustment of distance between the objective lens and the specimen when a "clear image" of the specimen is seen through the eyepiece.
Focusing
28
The image of an object seen in microscopic field.
Virtual or Floating image
29
The bright circular illuminated area as seen through the ocular eyepiece.
Microscopic Field
30
The distance between the front lens of the objective lens and top of the cover glass wen the specimen is in focus.
Working Distance
31
The angle or cone of light rays capable of entering the front lens of the objective from a point in the object.
Angular Aperture
32
It pertains to the thickness of the object that may be seen at one time under focus.
Depth of Focus
33
A measurement of the ability of the condenser and the objective lens to gather light.
Numerical Aperture
34
An ability of the lens to clearly separate or distinguish two points of two lines individually in the image
Resolving Power
35
It refers to the objectives and the eyepiece where practically no change in focus as to be made when one objective is substituted for another.
Parfocal
36
It is the bending of light rays away from the objective lens when light passes from the glass of the microscope slide to the air.
Refraction
37
The V-shaped or u-shaped structure that supports the whole instrument.
Base
38
The vertical extension of the base to which the arm is attache.
Pillar
39
The curved basic part of the microscope to which the base, body, and stage are attached.
Arm
40
Is the movable parts which facilitate titling of the microscope
Inclination Joints
41
A platform upon which the slide containing the specimen is placed. It is provided with a central aperture that allows light to pass from below to the object of the study.
Stage
42
A pair of metals that holds the slide in place. Some microscopes are provided with a mechanical stage which moves the specimen forward, backward, or sideward by means of a mechanical screw.
Stage clips
43
A hollow cylinder in front of the upper part of the arm that serves as housing for the lenses. It serves as a passageway of light from the objective to the eye piece.
Body tube
44
The smaller cylinder is attached to the top of the body tube and holds the ocular or eye piece.
Draw Tube
45
A fixed plate attached to the base of the body tube and situated above the revolving nosepiece. It protects objectives from dust and dirt.
Dust shield
46
A rotary head attached to the base of the body tube and holds the objectives/ It also facilitates the shifting of the objectives.
Revolving nosepiece
47
These are upper, large knobs used for faster movement of the body tube when focusing the low power objective
Coarse Adjustment Knobs or Screws
48
These are the lower, smaller knobs used for the final focusing under high power objective and viewing at different leves.
Fine Adjustment Knob
49
Usually two faced where one surface is plain or flat and concave on the other side. It is used to reflect light through the object, lens and into the eyes.
Mirror
50
An optical part under the stage to which is either fixed or provided with adjustment for lowering or raising until the field is evenly illuminated.
Abbe Condenser
51
A plate that is supported with numerous apertures of varying size to regulate the amount of light that enters the condenser.
Iris Diaphragm
52
The detachable tube situated on the top of the draw tube equipped with one set of lenses that magnifies the object several times.
Eyepiece or Ocular
53
The lens nearest to the objective being examined whereas the lens closest to the eye is termed the ocular (the eyepiece)
Objectives
54
The shortest cylinder with the widest lens opening but with the lowest magnification.
Scanner
55
The shortest tube with large lens opening which has a magnification of 10X. It is used to observe the general outline of the object.
Low power objective
56
The longer tube with smaller lens openign which has a magnification of 40x. It is used in observing the details of the specimen.
High power objective
57
The longest tube with a very small lens opening and has a magnification varying from 90-100x. Uses cedar wood oil.
Oil immersion objective

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