Microscope

Question 1

you only need a slight adjustment between objectives

Answer 1

Parfocal

Question 2

bright background and dark object

Answer 2

Bright field

Question 3

dark background and light object

Answer 3

Dark field

Question 4

Holds the ocular lenses, and revolving nosepiece; also serves as a support for the microscope; the other hand hold this part when carrying the microscope

Answer 4

Arm

Question 5

OIO - 100x - white HPO - 40x / 50x - blue LPO - 10x - yellow Scanner - 4x - red
Total Magnification = (objective) (ocular)

Answer 5

Objectives

Question 6

Where objectives are attached

Answer 6

Revolving nosepiece

Question 7

Serves as a support for the microscope; one hand is placed here when carrying a microscope

Answer 7

Base

Question 8

Has a magnification of 10x

Answer 8

Objective lenses

Question 9

Where the slides are places

Answer 9

Stage

Question 10

Holds the slides in place

Answer 10

Stage clips

Question 11

Found at the side of the microscope and are used for refining the image seen in the microscope

Answer 11

Course adjustment knob

Question 12

Adjusts light passing through

Answer 12

Diaphragm

Question 13

Adjusts light intensity

Answer 13

Rheostat

Question 14

Concentrates light entering the lens

Answer 14

Condenser

Question 15

Used for stained linearly oriented structures of living cells in tissue culture or fixed stained preparation.

Answer 15

Polarizing microscope

Question 16

It restricts the probing light to
preferred directions and orientations making it possible to detect the
presence of orderly arranged fibrous proteins or other arrays of long molecules.

Answer 16

Polarizing microscope

Question 17

Used for anisotropic substances — usually crystals (ex. uric acid, cholesterol)

Answer 17

Polarizing microscope

Question 18

Used in measurement applications. They provide

Answer 18

Confocal microscope

Question 19

A high-resolution image with all areas in focus throughout the field of view. Also enables non-contact non-destructive measurement of three dimensional shapes.

Answer 19

Confocal microscope

Question 20

The image is more focused thus, small area is focused

Answer 20

Confocal microscope

Question 21

Ultraviolet microscopes can image microscopic samples in the visible and the ultraviolet region.

Answer 21

Uv microscope

Question 22

Allows the visualization of naturally fluorescent substances (fluorescent- antibody (FA) technique, or immunofluorescence) or those that have been stained with a fluorochrome to produce an image

Answer 22

Fluorescence microscope

Question 23

Proides a 3D image showing very fine structural detial by splitting the ligh ray so that the beams pass through different areas of the specimen

Answer 23

Interference microscope

Question 24

Used to enhance the contrast in unstained or transparent samples to visualize living cells or tissues
- Cell additives: protoplasmic

Answer 24

Interference microscope

Question 25

- Note: Specimens should be transluscent, fixed and stained to provide contrast and to increase visualization Objects appear dark against a light background illuminated by a condenser. It is where stained tissues are examined with ordinary light passing through the preparation Image is inverted of the actual image

Answer 25

Bright field microscope

Question 26

- Enhances visualization of elements with low refractive. - Mineral elements, Spindle fibers - Confocal Microscope - The phase contrast microscope enables examination of unstained cells and tissues and is especially useful for living cells. - Function - Used in measurement applications. They provide - A high-resolution image with all areas in focus throughout the field of view. Also enables non-contact non-destructive measurement of three dimensional shapes. - A type of light microscopy that intensifies contrasts of transparent and colorless objects by influencing the optical path of light.

Answer 26

Phase contrast microscope

Question 27

- Uses electron beams - High resolution and magnification - Subcellular structures: organelles - In microbiology: visualization and study of viruses

Answer 27

Electron microscope

Question 28

Used to enhance visualization of specimens that cannot be seen easily with a bright-field microscope. It can be effectively used at high magnifications to photograph living bacteria, or at low magnifications to view and photograph cells, tissues, and whole mounts.

Answer 28

Dark field microscope