Microscope

Question 1

Who created the a simple microscope in the 1500s

Answer 1

Anton Vaughn Leeuwonhoek

Question 2

Coined term animalicule a.k.a microorganisms and is the father of microology or bacteriology

Answer 2

Anton Von Leeuwen Hoek

Question 3

Who saw chamber like shapes on plant cells and called them cells?

Answer 3

Robert Hooke

Question 4

What is microscopy?

Answer 4

Study of Microscopes

Question 5

This is a type of microscope that allows light rays to pass directly to the eyes without being blocked by opaque plate in condenser?

Answer 5

Brightfeild Microscope

Question 6

Components of a microscope

Answer 6

• Framework
• Stage
• Light Source
• Lens Systems
• Focusing Knobs
• Ocular Adjustments

Question 7

basic frame structure of a microscope

Answer 7

arm and base

Question 8

horizontal platform that supports the micro-
scope slide

Answer 8

stage

Question 9

clamping device used for holding and moving the slide around on the stage

Answer 9

stage adjustment

Question 10

it used to control the intensity of light

Answer 10

light intensity control

Question 11

Most microscopes use this for reducing light intensity

Answer 11

neutral density filter

Question 12

three lens systems of a microscope

Answer 12

oculars, the objectives, and the condenser

Question 13

Part of the Microscope that has a diopter adjustment ring. Can be ocular or binocular.

Answer 13

Eye Peice

Question 14

To make diopter adjustments, one focuses first with
the _______________ only.

Answer 14

right eye

Question 15

this part collects and directs the light from the lamp to the slide being studied

Answer 15

condenser

Question 16

regulates the amount of light that reaches the slide

Answer 16

diaphragm

Question 17

they are used for bringing objects into focus when studying an object on a slide

Answer 17

coarse adjustment and fine adjustment knob

Question 18

What objective lens should we always start with?

Answer 18

Scanner

Question 19

How do we find the total magnification?

Answer 19

(Magnification of Objective lens) x (Magnification of Ocular Lens) = Total Magnification

Question 20

For any light microscope, the limit of resolution
is about

Answer 20

0.2 μm

Question 21

What is the ideal magnification for the ocular lens?

Answer 21

10x

Question 22

laboratory microscopes have magnifications of 10x, 40x, and 100x. Name the three objective lens in the same order

Answer 22

low-power, high-dry, and oil immersion

Question 23

How do you transport a microscope?

Answer 23

Have one hand each on the arm and the base

Question 24

How do we take care of a microscope?

Answer 24

• keep workstation clean
• prevent any electric cords from dangling
• use paper lens or liquids like ethyl alchohol to clean the lens and objective lens
• place dust cover or in a cabinet with a lamp to prevent moisture to prevent fungus growth

Question 25

What are the characteristic of a good quality Brightfeild microscope?

Answer 25

Parfocal and Parcentral

Question 26

ability to completely separate two objects in a microscopic field.

Answer 26

resolving power

Question 27

mathematical expression that describes how the condenser lens concentrates and focuses the light rays from the light source

Answer 27

Numerical aperture

Question 28

range of distance in front of and behind a focused image within which other objects will appear clear and sharply defined

Answer 28

Depth of field

Question 29

it should be placed over the light source because the shorter wavelength of the resulting light will provide maximum resolution. what is it called?

Answer 29

blue filter

Question 30

- used between the slide and the 100 objective lens - special oil that has the same refractive index as glass.

Answer 30

Immersion oil

Question 31

powerful solvent that could possibly dissolve the lens mounting cement in some objective lenses if it were used too liberally

Answer 31

Acetone

Question 32

Various liquids can be used for cleaning microscope lenses like

Answer 32

Green soap with warm water, Xylene, Alcohol and acetone