microscope

Question 1

what is magnificiation

Answer 1

the number of times an image can be largened, compared to the real size of the object

Question 2

what is a photomicrograph

Answer 2

photograph made by optical microscope

Question 3

what is electron micrograph

Answer 3

photograph of an image by electron microscope

Question 4

what is resolution

Answer 4

clarity of an image
ability to distinguish between two separate points on an image

Question 5

what are the advantages of a light microscope

Answer 5

cheap
easy to use
can study whole organisms
organisms can be live - can see live processes
simple preparation of slides

Question 6

what is magnification of a optical microscope

Answer 6

x1500

Question 7

what is the resolution of a optical microscope

Answer 7

200nm

Question 8

what is the resolution of a Transmission electron microscope

Answer 8

0.5

Question 9

what is magnification of a SEM

Answer 9

3-10nm

Question 10

what is magnification of sem

Answer 10

x500,000

Question 11

would the magnification of a tem be higher or lower than sem

Answer 11

higher, as resolution is higher

Question 12

what organelles can microscopes not view

Answer 12

ribosomes, lysosomes, lipids

Question 13

write the stages of using an optical microscope

Answer 13

-specimen on slide in clipped to stage

-rotate nose piece and pick lowest objective lens to view

-adjust coarse focus knob (big) until image is focused

• adjust fine focus until image clear

repeat with different objective lens magnifications

Question 14

what is the I AM equation

Answer 14

equation used to work out image size- actual size divided by magnification

Question 15

what equation do u use to calculate total magnification

Answer 15

total magnification- magnifying power of objective lens x magnification of eye piece lens

Question 16

what is a confocal microscope

Answer 16

laser scanning microscope

Question 17

what is good about confocal microscope

Answer 17

high resolution
can view different depths of a specimen
can view living specimen so can see live processes
can view in colour

Question 18

what is the wavelength of electrons used in electron microscope

Answer 18

0.004nm

Question 19

how are the specimens prepared to view an image

Answer 19

specimen must be dehydrated and stained

Question 20

what is used in electron microscopes to focus image

Answer 20

magnets, where as glass lenses focus images in light microscopes

Question 21

electrons must be fired in this microscope, where are they fired from

Answer 21

cathode

Question 22

are light microscopes in colour or black and white

Answer 22

colour

Question 23

are confocal microscopes in colour or black and white

Answer 23

colour

Question 24

are electron microscopes in colour or black and white

Answer 24

black and white

Question 25

are optical microscopes 2d or 3d

Answer 25

2d

Question 26

are confocal microscopes 2d or 3d

Answer 26

3d

Question 27

how do images produced by TEM and SEM differ

Answer 27

tem- inside of cell sem- surface of cell or outside image of cell

Question 28

explain the difference between the beam of electrons from a tem and sem

Answer 28

tem- beam passes through specimen sem- beam bounces off specimen

Question 29

what is used to stain specimens

Answer 29

metal salts

Question 30

is tem 2d or 3d

Answer 30

2d black and white

Question 31

are sem 2d or 3d

Answer 31

3d

Question 32

what equipment is used to measure a specimen on a microscope

Answer 32

eyepiece graticule

Question 33

what is differential staining

Answer 33

multiple stains used to differentiate between cell structures

Question 34

when drawing a low power plan, how should the drawing look

Answer 34

simple continuous lines, outline and label. for example, outline: label plasma membrane, cytoplasm

Question 35

when drawing a high power drawing what should yo do

Answer 35

draw specific cells, more specific than low power.

Question 36

what should be included in scientific drawings

Answer 36

continuous lines, labels, scale, magnification, no overlapping